| pubmed-article:18925769 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1554184 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:18925769 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:18925769 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:18925769 | pubmed:dateCreated | 2009-2-20 | lld:pubmed |
| pubmed-article:18925769 | pubmed:abstractText | Unavailability of fusion tags that possess both affinity and visualization properties is a hurdle for biomolecular research. Typically, either a choice is made between an affinity tag and a reporter tag or both are employed in tandem if a fusion can be made at both termini of the target biomolecule. In this work, we have developed a site-specific genetic fusion approach employing DsRed-Monomer, a red fluorescent protein, that provides for both affinity and reporter functionality in a single tag. As a proof-of-concept, two fusion proteins, bradykinin-DsRed-Monomer and calmodulin-DsRed-Monomer, were prepared for the study. These fusion proteins were purified using a copper-immobilized column based on the inherent copper-binding property of DsRed-Monomer. Spectroscopic characterization of fusion proteins and comparison with native DsRed-Monomer showed no effect of fusion on the properties of DsRed-Monomer. Further, bradykinin-DsRed-Monomer was employed in the development of a competitive fluorescence immunoassay for the peptide bradykinin. Calmodulin-DsRed-Monomer was used to detect binding of the calmodulin ligand chlorpromazine, based on a change in the fluorescence of DsRed-Monomer upon binding of chlorpromazine to calmodulin. The studies performed demonstrate the application of DsRed-Monomer as a dual function tag indicating the potential usefulness of DsRed-monomer in proteomics and biomolecular research. | lld:pubmed |
| pubmed-article:18925769 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18925769 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18925769 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18925769 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18925769 | pubmed:issn | 1520-4812 | lld:pubmed |
| pubmed-article:18925769 | pubmed:author | pubmed-author:DeeS ASA | lld:pubmed |
| pubmed-article:18925769 | pubmed:author | pubmed-author:ShresthaSS | lld:pubmed |
| pubmed-article:18925769 | pubmed:author | pubmed-author:GouldingA MAM | lld:pubmed |
| pubmed-article:18925769 | pubmed:author | pubmed-author:RahimiYY | lld:pubmed |
| pubmed-article:18925769 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18925769 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:18925769 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:18925769 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18925769 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18925769 | pubmed:pagination | 2113-9 | lld:pubmed |
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| pubmed-article:18925769 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18925769 | pubmed:articleTitle | Dual function labeling of biomolecules based on DsRed-Monomer. | lld:pubmed |
| pubmed-article:18925769 | pubmed:affiliation | Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202, USA. | lld:pubmed |
| pubmed-article:18925769 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18925769 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
