| pubmed-article:18924151 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0210994 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0919458 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C1704873 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0879392 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C1824489 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0596260 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:18924151 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:18924151 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18924151 | pubmed:dateCreated | 2008-12-8 | lld:pubmed |
| pubmed-article:18924151 | pubmed:abstractText | Cellular function is dependent on tight regulation of intracellular pH and numerous reports show cancer cells have abnormal pH values in the cytosol and organelles, such as lysosomes. 5-(and-6)-carboxyl seminaphthorhodafluor (SNARF-1) is a commonly used pH sensitive probe and was used here to determine cytosolic pH of HL-60 leukemia cells and a drug-resistant variant overexpressing multidrug-resistance related protein 1 (MRP1). Resistant cells accumulated significantly less SNARF-1 compared to parental cells but near control levels of probe accumulation were observed by preincubating cells with the specific MRP1 inhibitor MK571. Two new drug-resistant cell lines were generated following exposure to doxorubicin or daunorubicin and these upregulated MRP1 or P-glycoprotein expression, respectively. Experiments in these cells showed that reduced SNARF-1 accumulation was specific to MRP1 overexpression, as cells upregulating P-glycoprotein accumulated control levels of the probe. Confirmation that SNARF-1 is a MRP1 substrate was obtained using K562 and KG1a cells that have been shown to, respectively, constitutively express MRP1 and P-glycoprotein. Together, the data suggest that SNARF-1 is a substrate for MRP1 but not P-glycoprotein, and could therefore be used as a probe to distinguish between expression and activity of these 2 efflux proteins. Finally, we confirm that doxorubicin but not daunorubicin challenged MRP1 overexpressing HL-60 cells have elevated cytosolic pH. | lld:pubmed |
| pubmed-article:18924151 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18924151 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18924151 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18924151 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:18924151 | pubmed:issn | 1097-0215 | lld:pubmed |
| pubmed-article:18924151 | pubmed:author | pubmed-author:JinJingJ | lld:pubmed |
| pubmed-article:18924151 | pubmed:author | pubmed-author:JonesArwyn... | lld:pubmed |
| pubmed-article:18924151 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18924151 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:18924151 | pubmed:volume | 124 | lld:pubmed |
| pubmed-article:18924151 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18924151 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18924151 | pubmed:pagination | 233-8 | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:meshHeading | pubmed-meshheading:18924151... | lld:pubmed |
| pubmed-article:18924151 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:18924151 | pubmed:articleTitle | The pH sensitive probe 5-(and-6)-carboxyl seminaphthorhodafluor is a substrate for the multidrug resistance-related protein MRP1. | lld:pubmed |
| pubmed-article:18924151 | pubmed:affiliation | Welsh School of Pharmacy, Cardiff University, Cardiff, Wales, United Kingdom. | lld:pubmed |
| pubmed-article:18924151 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18924151 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:4363 | entrezgene:pubmed | pubmed-article:18924151 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:18924151 | lld:entrezgene |
