| pubmed-article:1883916 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C0205102 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C1173313 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1883916 | lifeskim:mentions | umls-concept:C0337112 | lld:lifeskim |
| pubmed-article:1883916 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1883916 | pubmed:dateCreated | 1991-10-7 | lld:pubmed |
| pubmed-article:1883916 | pubmed:abstractText | The interleukin 2 receptor is a multisubunit receptor known to consist of at least two IL-2 binding subunits, alpha and beta. We report here kinetic evidence defining the contribution of an affinity-modulating element(s) intimately involved in modulation of the ligand-binding affinity of the beta chain and alpha/beta complex. The principal effect of this modulating element on the beta chain is to slow the dissociation of IL-2 more than 150-fold and thus raise its low intrinsic IL-2 binding affinity (Kd = 70 nM) as defined in transfected fibroblast cells to the level observed in lymphoid cells (Kd = 1.2 nM). The alpha subunit also increases the ligand-binding affinity of the beta chain, although in this case principally by increasing the association rate constant more than 1200-fold. The additional effect of the affinity-modulating element on the alpha/beta complex is minimal with regards to the equilibrium binding affinity. It does, however, have a detectable 14-fold effect on slowing the IL-2 dissociation rate. The existence of multiple forms of IL-2 receptor complexes with widely varying ligand affinities and dissociation rates illustrates the need for careful evaluation of binding data in studies of receptor subunit composition and reconstitution. | lld:pubmed |
| pubmed-article:1883916 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1883916 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1883916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1883916 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1883916 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1883916 | pubmed:issn | 1056-5477 | lld:pubmed |
| pubmed-article:1883916 | pubmed:author | pubmed-author:TESTA RAR | lld:pubmed |
| pubmed-article:1883916 | pubmed:author | pubmed-author:FreimarkB DBD | lld:pubmed |
| pubmed-article:1883916 | pubmed:author | pubmed-author:RingheimG EGE | lld:pubmed |
| pubmed-article:1883916 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1883916 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:1883916 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1883916 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1883916 | pubmed:pagination | 219-24 | lld:pubmed |
| pubmed-article:1883916 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:meshHeading | pubmed-meshheading:1883916-... | lld:pubmed |
| pubmed-article:1883916 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1883916 | pubmed:articleTitle | Quantitative characterization of the intrinsic ligand-binding affinity of the interleukin 2 receptor beta chain and its modulation by the alpha chain and a second affinity-modulating element. | lld:pubmed |
| pubmed-article:1883916 | pubmed:affiliation | Central Research and Development, Glenolden Laboratory, DuPont, PA 19036. | lld:pubmed |
| pubmed-article:1883916 | pubmed:publicationType | Journal Article | lld:pubmed |
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