| pubmed-article:18835848 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C0242613 | lld:lifeskim |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C0011155 | lld:lifeskim |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C0439605 | lld:lifeskim |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C1158478 | lld:lifeskim |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:18835848 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:18835848 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:18835848 | pubmed:dateCreated | 2008-11-4 | lld:pubmed |
| pubmed-article:18835848 | pubmed:abstractText | In higher animal cells, the principal limitation of gene-targeting technology is the extremely low efficiency of targeted integration, which occurs three to four orders of magnitude less frequently than random integration. Assuming that random integration mechanistically involves non-homologous end-joining (NHEJ), inactivation of this pathway should reduce random integration and may enhance gene targeting. To test this possibility, we examined the frequencies of random and targeted integration in NHEJ-deficient chicken DT40 and human Nalm-6 cell lines. As expected, loss of NHEJ resulted in drastically reduced random integration in DT40 cells. Unexpectedly, however, this was not the case for Nalm-6 cells, indicating that NHEJ is not the sole mechanism of random integration. Nevertheless, we present evidence that NHEJ inactivation can lead to enhanced gene targeting through a reduction of random integration and/or an increase in targeted integration by homologous recombination. Most intriguingly, our results show that, in the absence of functional NHEJ, random integration of targeting vectors occurs more frequently than non-targeting vectors (harboring no or little homology to the host genome), implying that suppression of NHEJ-independent random integration events is needed to greatly enhance gene targeting in animal cells. | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18835848 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18835848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835848 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18835848 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18835848 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:KoyamaHidekiH | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:IshiiAyakoA | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:IshiiYasuyuki... | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:AdachiNoritak... | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:KurosawaAyaA | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:IiizumiSusumu... | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:SoSaireiS | lld:pubmed |
| pubmed-article:18835848 | pubmed:author | pubmed-author:ChikaraishiYu... | lld:pubmed |
| pubmed-article:18835848 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18835848 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:18835848 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18835848 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18835848 | pubmed:pagination | 6333-42 | lld:pubmed |
| pubmed-article:18835848 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
| pubmed-article:18835848 | pubmed:meshHeading | pubmed-meshheading:18835848... | lld:pubmed |
