| pubmed-article:18814192 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C0005768 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C0072980 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:18814192 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:18814192 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18814192 | pubmed:dateCreated | 2009-2-16 | lld:pubmed |
| pubmed-article:18814192 | pubmed:abstractText | Therapeutic drug monitoring of sirolimus (rapamycin) is important for immunosuppressive therapy in solid organ transplantation. We have developed a simple and reliable method for determining blood concentrations of sirolimus using reversed-phase HPLC with electrochemical detection (ECD). The E(2) potential was set at +900 mV. The potential of guard cell was set at +950 mV and that of the E(1) cell at +400 mV. The method was linear for a concentration range of 1-50 ng/mL when 0.5 mL blood was used. The correlation coefficients of all standard curves were greater than or equal to 0.999. The limit of detection was 0.5 ng/mL. The inter-assay precision ranged from 3.22 to 7.48%, and the coefficient of variation (CV) for a quality control sample at 10 ng/mL was 7.48% with a bias of 8.4% from the target value. The intra-assay precision ranged from 0.72 to 3.71%, and the CV for a quality control sample at 10 ng/mL was 0.72% with a bias of 6.8% from the target value. In a solid organ transplant recipient, trough concentrations of sirolimus were well within the analytic range of the HPLC/ECD procedure. The method described here is suitable for management of sirolimus therapy in solid organ transplantation. | lld:pubmed |
| pubmed-article:18814192 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18814192 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18814192 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18814192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18814192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18814192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18814192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18814192 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18814192 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:18814192 | pubmed:issn | 1099-0801 | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:MatsumotoKuni... | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:OhnoKeikoK | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:TodoSatoruS | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:ShimamuraTsuy... | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:FurukawaHiroy... | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:KishinoSatosh... | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:MochizukiNobu... | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:SukaEtsukoE | lld:pubmed |
| pubmed-article:18814192 | pubmed:author | pubmed-author:AkimotoOsamuO | lld:pubmed |
| pubmed-article:18814192 | pubmed:copyrightInfo | 2008 John Wiley & Sons, Ltd. | lld:pubmed |
| pubmed-article:18814192 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18814192 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:18814192 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18814192 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18814192 | pubmed:pagination | 267-72 | lld:pubmed |
| pubmed-article:18814192 | pubmed:meshHeading | pubmed-meshheading:18814192... | lld:pubmed |
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| pubmed-article:18814192 | pubmed:meshHeading | pubmed-meshheading:18814192... | lld:pubmed |
| pubmed-article:18814192 | pubmed:meshHeading | pubmed-meshheading:18814192... | lld:pubmed |
| pubmed-article:18814192 | pubmed:meshHeading | pubmed-meshheading:18814192... | lld:pubmed |
| pubmed-article:18814192 | pubmed:meshHeading | pubmed-meshheading:18814192... | lld:pubmed |
| pubmed-article:18814192 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:18814192 | pubmed:articleTitle | Liquid chromatographic method for the determination of sirolimus in blood using electrochemical detection. | lld:pubmed |
| pubmed-article:18814192 | pubmed:affiliation | Department of Medication Use Analysis and Clinical Research, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. | lld:pubmed |
| pubmed-article:18814192 | pubmed:publicationType | Journal Article | lld:pubmed |
