| pubmed-article:18803763 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0684249 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0439640 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:18803763 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:18803763 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18803763 | pubmed:dateCreated | 2008-9-22 | lld:pubmed |
| pubmed-article:18803763 | pubmed:abstractText | XAGE-1b is regarded as one of the most immunogenic antigens and the most promising targets for lung adenocarcinoma immunotherapy. In this study, we sought to determine whether monocyte-derived dendritic cells (DCs) pulsed with purified full-length XAGE-1b could induce specific cytotoxic T lymphocytes (CTLs) against tumour cells from patients with non-small cell lung cancer (NSCLC) in vitro. XAGE-1b mRNA expression was examined in primary cultures of lung cancer cells and normal lung epithelial cells established from fresh tissues surgically resected from 30 patients with NSCLC using reverse transcription-polymerase chain reaction (RT-PCR). XAGE-1b mRNA expression was observed in 11 of 18 (61.1%) adenocarcinomas and one of 12 (8.3%) lung cancers of other histological types (P = 0.015). The 246-base pairs XAGE-1b gene was inserted into a recombinant expression vector. Full-length XAGE-1b was then expressed in BL21 (DE3) Escherichia coli and purified by AKTA-fast performance liquid chromatography (FPLC). DCs generated from peripheral blood mononuclear cells were pulsed with XAGE-1b by incubation with the protein at an immature stage. The XAGE-1b-pulsed DCs induced CTLs following 14 days of co-culture. Finally, an adherent target detachment (ATD) assay was performed to test the cytotoxicity of the XAGE-1b-specific CTLs against cancer cells and normal lung epithelial cells. The XAGE-1b-specific CTLs had a stronger lytic effect on autologous XAGE-1b mRNA-positive cancer cells than on autologous XAGE-1b mRNA-negative cancer cells or allogenous XAGE-1b mRNA-positive cancer cells. The CTLs had no lytic activity against normal lung epithelial cells. These results can be used to develop simple and effective cancer/testis antigen-based immunotherapies for NSCLC. | lld:pubmed |
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| pubmed-article:18803763 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18803763 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18803763 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18803763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18803763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18803763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18803763 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18803763 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18803763 | pubmed:issn | 1365-2249 | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:ZhouQQ | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:WangZZ | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:HoD NDN | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:XuY BYB | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:AnS-JSJ | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:GuoA-LAL | lld:pubmed |
| pubmed-article:18803763 | pubmed:author | pubmed-author:YangS-QSQ | lld:pubmed |
| pubmed-article:18803763 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18803763 | pubmed:volume | 153 | lld:pubmed |
| pubmed-article:18803763 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18803763 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18803763 | pubmed:pagination | 392-400 | lld:pubmed |
| pubmed-article:18803763 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:18803763 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18803763 | pubmed:articleTitle | A dendritic cell-based tumour vaccine for lung cancer: full-length XAGE-1b protein-pulsed dendritic cells induce specific cytotoxic T lymphocytes in vitro. | lld:pubmed |
| pubmed-article:18803763 | pubmed:affiliation | Division of Pulmonary Oncology, Cancer Center and Lung Cancer Research Institute, Guangdong Provincial People's Hospital, Guangzhou, China. | lld:pubmed |
| pubmed-article:18803763 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18803763 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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