| pubmed-article:18765236 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0003402 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C2717940 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0050587 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0598309 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:18765236 | lifeskim:mentions | umls-concept:C0243264 | lld:lifeskim |
| pubmed-article:18765236 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:18765236 | pubmed:dateCreated | 2008-11-10 | lld:pubmed |
| pubmed-article:18765236 | pubmed:abstractText | The chemoprotective effect of hydroxytyrosol (HT), a strong antioxidant compound from extra virgin olive oil, against acrylamide (AA)-induced genotoxicity was investigated in a human hepatoma cell line, HepG2. The micronucleus test (MNT) assay was used to monitor genotoxicity. In MNT, we found that HT at all tested concentrations (12.5-50 microM) significantly reduced the micronuclei frequencies in a concentration-dependent manner caused by AA. In order to clarify the underlying mechanisms we measured the intracellular reactive oxygen species (ROS) formation using 2,7-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe. Intracellular glutathione (GSH) level was estimated by fluorometric methods. The rate-limiting enzyme in GSH synthesis is gamma-glutamylcysteine synthetase (gamma-GCS) and gamma-GCS was measured using Western blotting. The results showed that HT significantly concentration-dependent reduced the genotoxicity caused by AA. Furthermore, HT was able to reduce intracellular ROS formation and attenuate GSH depletion caused by AA in a concentration-dependent manner. It was also found that HT enhanced the expression of gamma-GCS in HepG2 cells treated with 10 mM AA using immunoblotting in a concentration-dependent manner. The results showed that HT reduced the AA-induced genotoxicity by decreasing the ROS level and increasing the GSH level. The data strongly suggest that HT have significant protective ability against AA-induced genotoxicity in vitro. | lld:pubmed |
| pubmed-article:18765236 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18765236 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18765236 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18765236 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18765236 | pubmed:issn | 1872-7786 | lld:pubmed |
| pubmed-article:18765236 | pubmed:author | pubmed-author:ZhangXiaomeiX | lld:pubmed |
| pubmed-article:18765236 | pubmed:author | pubmed-author:JiangLipingL | lld:pubmed |
| pubmed-article:18765236 | pubmed:author | pubmed-author:YoshimuraHiro... | lld:pubmed |
| pubmed-article:18765236 | pubmed:author | pubmed-author:ZhongLaifuL | lld:pubmed |
| pubmed-article:18765236 | pubmed:author | pubmed-author:GengChenyanC | lld:pubmed |
| pubmed-article:18765236 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18765236 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:18765236 | pubmed:volume | 176 | lld:pubmed |
| pubmed-article:18765236 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18765236 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18765236 | pubmed:pagination | 173-8 | lld:pubmed |
| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
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| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
| pubmed-article:18765236 | pubmed:meshHeading | pubmed-meshheading:18765236... | lld:pubmed |
| pubmed-article:18765236 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18765236 | pubmed:articleTitle | Inhibition of acrylamide genotoxicity in human liver-derived HepG2 cells by the antioxidant hydroxytyrosol. | lld:pubmed |
| pubmed-article:18765236 | pubmed:affiliation | Department of Toxicology, Dalian Medical University, 465 Zhongshan Road, Dalian 116027, Liaoning, China. | lld:pubmed |
| pubmed-article:18765236 | pubmed:publicationType | Journal Article | lld:pubmed |
