pubmed-article:18761082 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:18761082 | lifeskim:mentions | umls-concept:C0001992 | lld:lifeskim |
pubmed-article:18761082 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
pubmed-article:18761082 | lifeskim:mentions | umls-concept:C0752046 | lld:lifeskim |
pubmed-article:18761082 | lifeskim:mentions | umls-concept:C0441704 | lld:lifeskim |
pubmed-article:18761082 | pubmed:issue | 5-6 | lld:pubmed |
pubmed-article:18761082 | pubmed:dateCreated | 2008-11-28 | lld:pubmed |
pubmed-article:18761082 | pubmed:abstractText | A simple and non-expensive platform is critical to realize on-site SNP typing. In this study we typed an SNP existing at the 487th residue of human aldehyde dehydrogenase2 [wild: Glu (GAA); mutant: Lys (AAA)] using our unique isothermal DNA amplification method, ICAN and cycling probes. Both genotypes were identified by the naked eye using a non-expensive UV transilluminator as well as with real-time PCR apparatus or a fluorescence detector. Since ICAN does not need thermal cycling, a cost- and space-limiting factor when fabricating apparatus, the combination of ICAN and cycling probes will be able to realize affordable on-site SNP typing in the near future. | lld:pubmed |
pubmed-article:18761082 | pubmed:language | eng | lld:pubmed |
pubmed-article:18761082 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:18761082 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:18761082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:18761082 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:18761082 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:18761082 | pubmed:issn | 0890-8508 | lld:pubmed |
pubmed-article:18761082 | pubmed:author | pubmed-author:MukaiHiroyuki... | lld:pubmed |
pubmed-article:18761082 | pubmed:author | pubmed-author:AsadaKiyozoK | lld:pubmed |
pubmed-article:18761082 | pubmed:author | pubmed-author:KatoIkunoshin... | lld:pubmed |
pubmed-article:18761082 | pubmed:author | pubmed-author:TakedaOsamuO | lld:pubmed |
pubmed-article:18761082 | pubmed:author | pubmed-author:UsuiKanakoK | lld:pubmed |
pubmed-article:18761082 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:18761082 | pubmed:volume | 22 | lld:pubmed |
pubmed-article:18761082 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:18761082 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:18761082 | pubmed:pagination | 333-7 | lld:pubmed |
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pubmed-article:18761082 | pubmed:articleTitle | SNP typing of aldehyde dehydrogenase2 gene with Cycleave ICAN. | lld:pubmed |
pubmed-article:18761082 | pubmed:affiliation | Products Development Center, Takara Bio Inc., 2257, Noji, Kusatsu, Shiga 525-0055, Japan. | lld:pubmed |
pubmed-article:18761082 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:18761082 | pubmed:publicationType | Evaluation Studies | lld:pubmed |