| pubmed-article:18722102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18722102 | lifeskim:mentions | umls-concept:C0023690 | lld:lifeskim |
| pubmed-article:18722102 | lifeskim:mentions | umls-concept:C1708726 | lld:lifeskim |
| pubmed-article:18722102 | lifeskim:mentions | umls-concept:C0162735 | lld:lifeskim |
| pubmed-article:18722102 | lifeskim:mentions | umls-concept:C0752046 | lld:lifeskim |
| pubmed-article:18722102 | lifeskim:mentions | umls-concept:C0439794 | lld:lifeskim |
| pubmed-article:18722102 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18722102 | pubmed:dateCreated | 2008-10-20 | lld:pubmed |
| pubmed-article:18722102 | pubmed:abstractText | Detailed analyses of dense single nucleotide polymorphism (SNP) loci within rifampin-resistance determining region (RRDR) are very important for the early assessment of drug resistance of Mycobacterium tuberculosis. A strategy was developed here to specifically identify point mutations out of dense SNP loci by on-chip ligation of multiplexing probe-pairs (MPPs). A probe-pair combines a common probe with a discriminating probe which is covalently attached to a DNA chip. The common probe hybridizes to the discriminating probe via a unique "zip-code complement". The allele-specific part on the 3'-end of the discriminating probe becomes covalently ligated to the adjacent part on the 5'-end of the common probe if and only if a mutation is present. Thus upon zip-code recognition, the process of identifying a mutation of interest is entirely located into corresponding well on the chip. As a consequence, cross-reactions and biased competitive attachments to targets, both of which result from the presence of various multiplexing probes, are greatly minimized. Mutation detection was performed by direct visualization using enzyme-linked assay. The method was demonstrated with an initial set of 24 probe-pairs targeting 22 clinically meaningful mutations within an 81-bp RRDR. 130-bp fragments of the rpoB gene from 15 clinical isolates were identified and were in 100% agreement with results from independent sequencing. | lld:pubmed |
| pubmed-article:18722102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18722102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18722102 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18722102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18722102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18722102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18722102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18722102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18722102 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:18722102 | pubmed:issn | 1873-4235 | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:ZhangXian-EnX... | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:ZhouYa-FengYF | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:ZhangZhi-Ping... | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:EideA JAJ | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:BiLi-JunLJ | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:DengJiao-YuJY | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:WeiHong-PingH... | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:DengXiangX | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:CuiZong-Qiang... | lld:pubmed |
| pubmed-article:18722102 | pubmed:author | pubmed-author:GuoYong-ChaoY... | lld:pubmed |
| pubmed-article:18722102 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18722102 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:18722102 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:18722102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18722102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18722102 | pubmed:pagination | 818-24 | lld:pubmed |
| pubmed-article:18722102 | pubmed:dateRevised | 2009-7-14 | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
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| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:meshHeading | pubmed-meshheading:18722102... | lld:pubmed |
| pubmed-article:18722102 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18722102 | pubmed:articleTitle | On-chip ligation of multiplexing probe-pairs for identifying point mutations out of dense SNP loci. | lld:pubmed |
| pubmed-article:18722102 | pubmed:affiliation | State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Xiao Hong Shan, Wuchang, Wuhan 430071, PR China. | lld:pubmed |
| pubmed-article:18722102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18722102 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
