Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:18690413rdf:typepubmed:Citationlld:pubmed
pubmed-article:18690413lifeskim:mentionsumls-concept:C0021289lld:lifeskim
pubmed-article:18690413lifeskim:mentionsumls-concept:C0034721lld:lifeskim
pubmed-article:18690413lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:18690413lifeskim:mentionsumls-concept:C0225828lld:lifeskim
pubmed-article:18690413lifeskim:mentionsumls-concept:C0021701lld:lifeskim
pubmed-article:18690413lifeskim:mentionsumls-concept:C0020564lld:lifeskim
pubmed-article:18690413pubmed:issue1-2lld:pubmed
pubmed-article:18690413pubmed:dateCreated2008-12-10lld:pubmed
pubmed-article:18690413pubmed:abstractTextProlonged myocardial stretch typically leads to hypertrophy of cardiomyocytes. As integrins are cellular receptors of stretch, we hypothesize that integrin stimulation induces cardiomyocyte hypertrophy. Integrins of neonatal rat cardiomyocytes (NRCMs) were stimulated with a peptide containing the Arg-Gly-Asp (RGD) sequence for 24 h. For comparison, alpha(1)-adrenergic stimulation by phenylephrine (PE) for 24 h was applied. Saline-treated NRCMs were used as control. The hypertrophic response was quantified by measuring cell surface area (CSA). Phosphorylation of NO-synthase-1 (NOS1) was assessed by immunocytochemistry. CSA was increased by 38% (IQR 31-44%) with RGD and by 68% (IQR 64-84%) with PE versus control (both P < 0.001). NOS-1 phosphorylation was increased by 61% with RGD and by 21% with PE versus control (both P < 0.01). A general NOS-inhibitor (L-NAME) inhibited RGD-induced hypertrophy completely, but had no significant effect on PE-induced hypertrophy. Administration of NO-donor to NRCMs co-incubated with RGD + L-NAME partly restored hypertrophy (to 62% of the hypertrophic effect of RGD alone), but had no effect if incubated with PE + L-NAME. Ryanodine and BAPTA-AM inhibited RGD-induced hypertrophy completely but not that induced by PE. Integrin stimulation of NRCMs by RGD leads to hypertrophy, likely by activation of NOS-1. Abrogation of RGD-induced hypertrophic response upon NOS-inhibition and rescue of this hypertrophic effect by NO-donor suggest that integrin stimulation-induced hypertrophy of NRCMs is NO-dependent.lld:pubmed
pubmed-article:18690413pubmed:languageenglld:pubmed
pubmed-article:18690413pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:citationSubsetIMlld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18690413pubmed:statusMEDLINElld:pubmed
pubmed-article:18690413pubmed:monthJanlld:pubmed
pubmed-article:18690413pubmed:issn1573-4919lld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:van der...lld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:van der...lld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:SchalijM JMJlld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:UmarSSlld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:AtsmaD EDElld:pubmed
pubmed-article:18690413pubmed:authorpubmed-author:van der...lld:pubmed
pubmed-article:18690413pubmed:issnTypeElectroniclld:pubmed
pubmed-article:18690413pubmed:volume320lld:pubmed
pubmed-article:18690413pubmed:ownerNLMlld:pubmed
pubmed-article:18690413pubmed:authorsCompleteYlld:pubmed
pubmed-article:18690413pubmed:pagination75-84lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:meshHeadingpubmed-meshheading:18690413...lld:pubmed
pubmed-article:18690413pubmed:year2009lld:pubmed
pubmed-article:18690413pubmed:articleTitleIntegrin stimulation-induced hypertrophy in neonatal rat cardiomyocytes is NO-dependent.lld:pubmed
pubmed-article:18690413pubmed:affiliationDepartment of Cardiology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands. S.umar@lumc.nllld:pubmed
pubmed-article:18690413pubmed:publicationTypeJournal Articlelld:pubmed