| pubmed-article:18688043 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C0026820 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C1257975 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C0389995 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C1335631 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:18688043 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:18688043 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:18688043 | pubmed:dateCreated | 2008-9-17 | lld:pubmed |
| pubmed-article:18688043 | pubmed:abstractText | Sphingosylphosphorylcholine (SPC) induces differentiation of human adipose tissue-derived mesenchymal stem cells (hADSCs) to smooth muscle cells (SMCs). In the present study, we characterized contractile and ion channel properties of SMCs differentiated from hADSCs (hADSC-SMCs) as a result of SPC treatment, and we investigated the molecular mechanisms involved in the SPC-induced differentiation. Using in vitro collagen gel lattice contraction and whole cell patch clamp, we showed that the hADSC-SMCs expressed functional L-type voltage-gated Ca2+ channels and contractile activities in response to KCl, carbachol, and the L-type Ca2+ channel opener Bay K8644, whereas the L-type Ca2+ channel blocker nifedipine abrogated the contractility of hADSC-SMCs. Furthermore, hADSC-SMCs expressed functional big conductance Ca2+-activated K+ (BK(Ca)) channels, and the BK(Ca) channel blocker iberiotoxin potentiated the Bay K8644-stimulated contractility of the hADSC-SMCs, indicating that these cells exhibited SMC-like contractile characteristics. SPC activated RhoA in hADSCs and pretreatment with the Rho kinase inhibitor Y27632 or by overexpression of dominant-negative mutants of RhoA or Rho kinase completely abrogated the SPC-induced differentiation of hADSCs into SMCs. SPC also increased the expression levels of myocardin-related transcription factor (MRTF)-A, a transcription factor involved in smooth muscle differentiation, in hADSCs. Small interference RNA-mediated depletion of endogenous MRTF-A abolished the SPC-induced differentiation of hADSCs into SMCs. Furthermore, SPC promoted nuclear translocation of MRTF-A, and pharmacological inhibition of Rho kinase blocked this effect. These results suggest that SPC induced differentiation of hADSCs into contractile SMCs through a mechanism involving RhoA/Rho kinase-dependent nuclear translocation of MRTF-A. | lld:pubmed |
| pubmed-article:18688043 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18688043 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18688043 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18688043 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18688043 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18688043 | pubmed:issn | 1524-4571 | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:KimYoung MiYM | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:KimJae HoJH | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:HanJinJ | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:JeonEun SuES | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:ParkWon SunWS | lld:pubmed |
| pubmed-article:18688043 | pubmed:author | pubmed-author:LeeMi JeongMJ | lld:pubmed |
| pubmed-article:18688043 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18688043 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:18688043 | pubmed:volume | 103 | lld:pubmed |
| pubmed-article:18688043 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18688043 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18688043 | pubmed:pagination | 635-42 | lld:pubmed |
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| pubmed-article:18688043 | pubmed:meshHeading | pubmed-meshheading:18688043... | lld:pubmed |
| pubmed-article:18688043 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18688043 | pubmed:articleTitle | A Rho kinase/myocardin-related transcription factor-A-dependent mechanism underlies the sphingosylphosphorylcholine-induced differentiation of mesenchymal stem cells into contractile smooth muscle cells. | lld:pubmed |
| pubmed-article:18688043 | pubmed:affiliation | Department of Physiology, Pusan National University College of Medicine, Busan 602-739, Republic of Korea. | lld:pubmed |
| pubmed-article:18688043 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18688043 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:18688043 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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