| pubmed-article:18675405 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C0086140 | lld:lifeskim |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:18675405 | lifeskim:mentions | umls-concept:C0057622 | lld:lifeskim |
| pubmed-article:18675405 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:18675405 | pubmed:dateCreated | 2008-8-25 | lld:pubmed |
| pubmed-article:18675405 | pubmed:abstractText | The carbonyl polysaccharide, keto-dextran, was synthesized by the regioselective oxidation of sucrose and by the subsequent transfer reaction of the oxidized sucrose. The regioselective oxidation of sucrose was performed by bioconversion with pyranose-2-oxidase (EC 1.1.3.10). After 24h, the conversion percentage of sucrose into keto-sucrose was 100% as determined by a colorimetric method with dinitrophenylhydrazine. Converted keto-sucrose was polymerized to keto-dextran by dextransucrase (EC 2.4.1.5). Polymerization of keto-dextran was confirmed by the increase in molecular weight and amount of keto-dextran produced. The amount of keto-dextran produced decreased to 80% of the amount of dextran produced owing to the substrate recognition of DSase. From a Lineweaver-Burk reciprocal plot, the Michaelis constants for sucrose and keto-sucrose were 4.6 mmol L(-1) and 14.0 mmol L(-1), respectively. The keto-dextran had a carbonyl group in all glucose units. | lld:pubmed |
| pubmed-article:18675405 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18675405 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18675405 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18675405 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18675405 | pubmed:issn | 0008-6215 | lld:pubmed |
| pubmed-article:18675405 | pubmed:author | pubmed-author:HaradaHiroyuk... | lld:pubmed |
| pubmed-article:18675405 | pubmed:author | pubmed-author:KawakitaHidet... | lld:pubmed |
| pubmed-article:18675405 | pubmed:author | pubmed-author:SetoHirokazuH | lld:pubmed |
| pubmed-article:18675405 | pubmed:author | pubmed-author:InoueKatsutos... | lld:pubmed |
| pubmed-article:18675405 | pubmed:author | pubmed-author:OhtoKeisukeK | lld:pubmed |
| pubmed-article:18675405 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18675405 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:18675405 | pubmed:volume | 343 | lld:pubmed |
| pubmed-article:18675405 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18675405 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18675405 | pubmed:pagination | 2417-21 | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:meshHeading | pubmed-meshheading:18675405... | lld:pubmed |
| pubmed-article:18675405 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18675405 | pubmed:articleTitle | Novel carbonyl-group-containing dextran synthesis by pyranose-2-oxidase and dextransucrase. | lld:pubmed |
| pubmed-article:18675405 | pubmed:affiliation | Faculty of Science and Engineering, Department of Applied Chemistry, Saga University, 1-Honjo Saga 840-8502, Japan. | lld:pubmed |
| pubmed-article:18675405 | pubmed:publicationType | Journal Article | lld:pubmed |
