| pubmed-article:18652770 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18652770 | lifeskim:mentions | umls-concept:C0684249 | lld:lifeskim |
| pubmed-article:18652770 | lifeskim:mentions | umls-concept:C0664336 | lld:lifeskim |
| pubmed-article:18652770 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:18652770 | lifeskim:mentions | umls-concept:C2347610 | lld:lifeskim |
| pubmed-article:18652770 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:18652770 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18652770 | pubmed:dateCreated | 2008-7-25 | lld:pubmed |
| pubmed-article:18652770 | pubmed:abstractText | We investigated the difference in survivin expression between a multidrug-resistant lung cancer cell line (H460/cDDP) and its parental counterpart (H460) and the influence of siRNA targeting survivin on the chemosensitivity of H460/cDDP. SiRNA targeting survivin was transfected into H460/cDDP cells using a liposome approach. Survivin mRNA and protein expression were significantly higher in H460/cDDP than H460 cells. The median inhibitory concentrations (IC(50)s) for cisplatin and paclitaxol in vitro against H460/cDDP cells were significantly lower in cells treated with survivin-specific siRNA than in control cells. Apoptosis and cleaved caspase-3 expression were analysed using annexin V and Western blotting, respectively, and showed a significant increase in apoptosis after treatment with the chemotherapeutic agents plus specific siRNA. Specific siRNA sensitized H460/cDDP cells to both cisplatin and paclitaxol. Thus, survivin appears to participate in the multidrug resistance mechanism of H460/cDDP cells and siRNA targeting survivin has the potential to increase the sensitivity of drug-resistant cancer cells to anticancer drugs. | lld:pubmed |
| pubmed-article:18652770 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18652770 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18652770 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18652770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18652770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18652770 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18652770 | pubmed:issn | 0300-0605 | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:ORRS HSH | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:WangGG | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:YangHH | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:WenJJ | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:ZhangXX | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:ZhengBB | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:GEEMM | lld:pubmed |
| pubmed-article:18652770 | pubmed:author | pubmed-author:HuangW-ZWZ | lld:pubmed |
| pubmed-article:18652770 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18652770 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:18652770 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18652770 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18652770 | pubmed:pagination | 734-47 | lld:pubmed |
| pubmed-article:18652770 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:18652770 | pubmed:articleTitle | Influence of SiRNA targeting survivin on chemosensitivity of H460/cDDP lung cancer cells. | lld:pubmed |
| pubmed-article:18652770 | pubmed:affiliation | Department of Thoracic Oncology, Cancer Centre, Sun Yat-Sen University, State Key Laboratory of Oncology in Southern China, Guangzhou, PR China. | lld:pubmed |
| pubmed-article:18652770 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18652770 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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