Linked Life Data

18646003

Source:http://linkedlifedata.com/resource/pubmed/id/18646003

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pubmed-article:18646003pubmed:dateCreated2008-10-13lld:pubmed
pubmed-article:18646003pubmed:abstractTextBesides flavan-3-ols, a family of N-phenylpropenoyl-L-amino acids (NPAs) has been recently identified as polyphenol/amino acid conjugates in the seeds of Theobroma cacao as well as in a variety of herbal drugs. Stimulated by reports on their biological activity, the purpose of this study was to investigate if these amides are absorbed by healthy volunteers after administration of a cocoa drink. For the first time, 12 NPAs were quantified in human urine by means of a stable isotope dilution analysis with LC-MS/MS (MRM) detection. A maximum amount was found in the urine taken 2 h after the cocoa consumption. The highest absolute amount of NPAs excreted with the urine was found for N-[4'-hydroxy-(E)-cinnamoyl]-L-aspartic acid (5), but the highest recovery rate (57.3 and 22.8%), that means the percentage amount of ingested amides excreted with the urine, were determined for N-[4'-hydroxy-(E)-cinnamoyl]-L-glutamic acid (6) and N-[4'-hydroxy-3'-methoxy-(E)-cinnamoyl]-L-tyrosine (13). In order to gain first insights into the NPA metabolism in vivo, urine samples were analyzed by LC-MS/MS before and after beta-glucuronidase/sulfatase treatment. As independent of the enzyme treatment the same NPA amounts were found in urine, there is strong evidence that these amides are metabolized neither via their O-glucuronides nor their O-sulfates. In order to screen for caffeic acid O-glucuronides as potential NPA metabolites, urine samples were screened by means of LC-MS/MS for caffeic acid 3-O-beta-D-glucuronide and 4-O-beta-D-glucuronide. But not even trace amounts of one of these glucuronides were detectable, thus excluding them as major NPA metabolites and underlining the importance of future investigations on a potential O-methylation or reduction of the N-phenylpropenoyl moiety in NPAs.lld:pubmed
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pubmed-article:18646003pubmed:monthOctlld:pubmed
pubmed-article:18646003pubmed:issn1613-4133lld:pubmed
pubmed-article:18646003pubmed:authorpubmed-author:HofmannThomas...lld:pubmed
pubmed-article:18646003pubmed:authorpubmed-author:HenselAndreas...lld:pubmed
pubmed-article:18646003pubmed:authorpubmed-author:StarkTimoTlld:pubmed
pubmed-article:18646003pubmed:authorpubmed-author:KellerDaniela...lld:pubmed
pubmed-article:18646003pubmed:authorpubmed-author:LangRomanRlld:pubmed
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pubmed-article:18646003pubmed:volume52lld:pubmed
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pubmed-article:18646003pubmed:pagination1201-14lld:pubmed
pubmed-article:18646003pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:18646003pubmed:year2008lld:pubmed
pubmed-article:18646003pubmed:articleTitleAbsorption of N-phenylpropenoyl-L-amino acids in healthy humans by oral administration of cocoa (Theobroma cacao).lld:pubmed
pubmed-article:18646003pubmed:affiliationLehrstuhl für Lebensmittelchemie und Molekulare Sensorik, Technische Universität München, Freising, Germany.lld:pubmed
pubmed-article:18646003pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:18646003pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed