pubmed-article:1862081 | rdf:type | pubmed:Citation | lld:pubmed |
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pubmed-article:1862081 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
pubmed-article:1862081 | pubmed:issue | 15 | lld:pubmed |
pubmed-article:1862081 | pubmed:dateCreated | 1991-9-4 | lld:pubmed |
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pubmed-article:1862081 | pubmed:abstractText | Carotenoids are orange, yellow, or red photo-protective pigments present in all plastids. The first carotenoid of the pathway is phytoene, a colorless compound that is converted into colored carotenoids through a series of desaturation reactions. Genes coding for carotenoid desaturases have been cloned from microbes but not from plants. We report the cloning of a cDNA for pds1, a soybean (Glycine max) gene that, based on a complementation assay using the photosynthetic bacterium Rhodobacter capsulatus, codes for an enzyme that catalyzes the two desaturation reactions that convert phytoene into zeta-carotene, a yellow carotenoid. The 2281-base-pair cDNA clone analyzed contains an open reading frame with the capacity to code for a 572-residue protein of predicted Mr 63,851. Alignment of the deduced Pds1 peptide sequence with the sequences of fungal and bacterial carotenoid desaturases revealed conservation of several amino acid residues, including a dinucleotide-binding motif that could mediate binding to FAD. The Pds1 protein is synthesized in vitro as a precursor that, upon import into isolated chloroplasts, is processed to a smaller mature form. Hybridization of the pds1 cDNA to genomic blots indicated that this gene is a member of a low-copy-number gene family. One of these loci was genetically mapped using restriction fragment length polymorphisms between Glycine max and Glycine soja. We conclude that pds1 is a nuclear gene encoding a phytoene desaturase enzyme that, as its microbial counterparts, contains sequence motifs characteristic of flavoproteins. | lld:pubmed |
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pubmed-article:1862081 | pubmed:language | eng | lld:pubmed |
pubmed-article:1862081 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1862081 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1862081 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1862081 | pubmed:month | Aug | lld:pubmed |
pubmed-article:1862081 | pubmed:issn | 0027-8424 | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:HirschbergJJ | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:ScolnikP APA | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:PeckerII | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:ViitanenP VPV | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:ChamovitzDD | lld:pubmed |
pubmed-article:1862081 | pubmed:author | pubmed-author:BartleyG EGE | lld:pubmed |
pubmed-article:1862081 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1862081 | pubmed:day | 1 | lld:pubmed |
pubmed-article:1862081 | pubmed:volume | 88 | lld:pubmed |
pubmed-article:1862081 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1862081 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1862081 | pubmed:pagination | 6532-6 | lld:pubmed |
pubmed-article:1862081 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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pubmed-article:1862081 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:1862081 | pubmed:articleTitle | Molecular cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. | lld:pubmed |
pubmed-article:1862081 | pubmed:affiliation | Du Pont Central Research and Development, Wilmington, DE 19880-0402. | lld:pubmed |
pubmed-article:1862081 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1862081 | pubmed:publicationType | Comparative Study | lld:pubmed |
entrez-gene:547970 | entrezgene:pubmed | pubmed-article:1862081 | lld:entrezgene |
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