| pubmed-article:1861534 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C0018183 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C1517806 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C0439677 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C1157216 | lld:lifeskim |
| pubmed-article:1861534 | lifeskim:mentions | umls-concept:C0449774 | lld:lifeskim |
| pubmed-article:1861534 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1861534 | pubmed:dateCreated | 1991-9-4 | lld:pubmed |
| pubmed-article:1861534 | pubmed:abstractText | The production of sulfated proteoglycans was compared in mature peripheral blood granulocytes and monocytes and HL-60 promyelocytic leukemia cells. We found that HL-60 cells synthesized 5-10 times more proteoglycans than peripheral blood leukocytes. Differentiation of HL-60 cells toward mature monocytes by treatment with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or towards granulocytes by treatment with retinoic acid or dibutyryl cyclic adenosine-3',5'-monophosphate (dbcAMP) resulted in a small (20-30%) decrease in sulfated proteoglycan biosynthesis. Chondroitin sulfate was found to be the predominant proteoglycan produced by monocytes, PMN and undifferentiated HL-60 cells. Differentiated HL-60 cells produced chondroitin sulfate as well as sulfated proteoglycans sensitive to nitrous acid degradation. Similar results were observed in TPA, dbcAMP and retinoic acid differentiated HL-60 cells indicating that the changes in proteoglycan biosynthesis observed were independent of the developmental pathway. Using specific monoclonal antibodies and flow cytometry, we also found that HL-60 cells and monocytes produced chondroitin-4-sulfate, chondroitin-6-sulfate and chondroitin-O-sulfate while PMN only produced chondroitin-4-sulfate. In addition, although there were no significant differences in antibody binding to undifferentiated and differentiated HL-60 cells, the tumor cells bound 5-20 times more of the antibodies than the peripheral blood leukocytes. Our data demonstrate that sulfated proteoglycan production by HL-60 cells is distinct from PMN and monocytes. In addition, the fact that differentiated HL-60 cells continue to synthesize larger amounts of the proteoglycans than the peripheral blood leukocytes indicates these cells have not completely matured. | lld:pubmed |
| pubmed-article:1861534 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1861534 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1861534 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1861534 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1861534 | pubmed:issn | 0145-2126 | lld:pubmed |
| pubmed-article:1861534 | pubmed:author | pubmed-author:LaskinJ DJD | lld:pubmed |
| pubmed-article:1861534 | pubmed:author | pubmed-author:LaskinD LDL | lld:pubmed |
| pubmed-article:1861534 | pubmed:author | pubmed-author:SirakA AAA | lld:pubmed |
| pubmed-article:1861534 | pubmed:author | pubmed-author:DokidisAA | lld:pubmed |
| pubmed-article:1861534 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1861534 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:1861534 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1861534 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1861534 | pubmed:pagination | 515-23 | lld:pubmed |
| pubmed-article:1861534 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:meshHeading | pubmed-meshheading:1861534-... | lld:pubmed |
| pubmed-article:1861534 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1861534 | pubmed:articleTitle | Distinct patterns of sulfated proteoglycan biosynthesis in human monocytes, granulocytes and myeloid leukemic cells. | lld:pubmed |
| pubmed-article:1861534 | pubmed:affiliation | Department of Environmental and Community Medicine, UMDNJ-Robert Wood Johnson Medical School, Rutgers University, Piscataway 08854. | lld:pubmed |
| pubmed-article:1861534 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1861534 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1861534 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1861534 | lld:pubmed |
