Source:http://linkedlifedata.com/resource/pubmed/id/18613136
| Subject | Predicate | Object | Context |
|---|---|---|---|
| pubmed-article:18613136 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18613136 | lifeskim:mentions | umls-concept:C1039861 | lld:lifeskim |
| pubmed-article:18613136 | lifeskim:mentions | umls-concept:C0031001 | lld:lifeskim |
| pubmed-article:18613136 | lifeskim:mentions | umls-concept:C0679199 | lld:lifeskim |
| pubmed-article:18613136 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:18613136 | lifeskim:mentions | umls-concept:C0073590 | lld:lifeskim |
| pubmed-article:18613136 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:18613136 | pubmed:dateCreated | 2008-7-9 | lld:pubmed |
| pubmed-article:18613136 | pubmed:abstractText | The production of an intracellular secondary metabolite rosmarinic acid (RA) by plant cell suspensions of Anchusa officinalis cultivated with intermittent medium exchange is investigated. Initially, a two-stage perfusion culture method was employed. After being cultured in the batch mode for ca. 6 days in B5 medium plus 3% sucrose, 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and 0.1 mg/L kinetin (2,4-D B5 medium), Anchusa culture was cultivated to high cell density by perfusion during the growth stage using a hormone-free Gamborg B5 medium supplemented with 6% sucrose. This was followed by a production stage, in which a complete medium exchange into B5 medium plus 3% sucrose and 0.25 mg/L naphthleneacetic acid (NAA) was conducted. The two-stage perfusion culture had a higher maximum culture RA concentration but a lower RA content per cell than the batch stock culture maintained in the 2,4-D B5 medium. Higher culture RA concentration was due primarily to high cell density. The high packed cell volume, however, seemed to reduce the synergistic effect of NAA on RA synthesis. Subsequently, a single-stage perfusion culture method was investigated. The best result was obtained by growing the culture in the batch mode for ca. 10 days using B5 medium supplemented with 3% sucrose and 0.25 mg/L NAA, followed by perfusing the culture with B5 medium plus 6% sucrose and 0.25 mg/L NAA at a constant perfusion rate of 0.1/day. A maximum cell dry weight of 35 g/L and a RA concentration of almost 4 g/L were achieved. This is the highest RA concentration ever reported in the Anchusa culture. | lld:pubmed |
| pubmed-article:18613136 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18613136 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18613136 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:18613136 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18613136 | pubmed:issn | 0006-3592 | lld:pubmed |
| pubmed-article:18613136 | pubmed:author | pubmed-author:RauJJ | lld:pubmed |
| pubmed-article:18613136 | pubmed:author | pubmed-author:LeuJJ | lld:pubmed |
| pubmed-article:18613136 | pubmed:author | pubmed-author:SuL YLY | lld:pubmed |
| pubmed-article:18613136 | pubmed:copyrightInfo | (c) 1993 John Wiley & Sons, Inc. | lld:pubmed |
| pubmed-article:18613136 | pubmed:issnType | lld:pubmed | |
| pubmed-article:18613136 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:18613136 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:18613136 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18613136 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18613136 | pubmed:pagination | 884-90 | lld:pubmed |
| pubmed-article:18613136 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:18613136 | pubmed:articleTitle | Perfusion strategy for rosmarinic acid production by Anchusa officinalis. | lld:pubmed |
| pubmed-article:18613136 | pubmed:affiliation | Biochemical Engineering Laboratory, Department of Agricultural Engineering, University of Hawaii, Honolulu, Hawaii 96822, USA. | lld:pubmed |
| pubmed-article:18613136 | pubmed:publicationType | Journal Article | lld:pubmed |