| pubmed-article:18589832 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18589832 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:18589832 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:18589832 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:18589832 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:18589832 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:18589832 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18589832 | pubmed:dateCreated | 2008-6-30 | lld:pubmed |
| pubmed-article:18589832 | pubmed:abstractText | Nerve growth factor (NGF) promotes neuronal survival and differentiation and stimulates neurite outgrowth. NGF is synthesized as a precursor-proNGF in vivo. In this paper, a pET-proNGF prokaryocyte expression vector was constructed and transformed into E. coli BL21(DE3)pLysS. The proNGF was expressed in the form of non-active aggregated monomer in E. coli after induction with IPTG. SDS-PAGE revealed the proNGF expression product had a Mr.30.2 kD. Western blotting analysis showed that the protein had good antigenicity. Fusion protein was successfully purified by Ni2+-NTA affinity chromatography and cleaved by Enterokinase and 13.1 mg proNGF was obtained from 100 mL cell culture in a typical experiment. The protein was dialyzed in a redox system containing reduced and oxidized glutathione. RP-HPLC was used to analysis the result of the refolding. The refolded proNGF protein can induce neurite outgrowth of PC12 cells, which indicated that pro-form of NGF we obtained had biological activity. | lld:pubmed |
| pubmed-article:18589832 | pubmed:language | chi | lld:pubmed |
| pubmed-article:18589832 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18589832 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18589832 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18589832 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18589832 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18589832 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18589832 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18589832 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:18589832 | pubmed:issn | 1000-3061 | lld:pubmed |
| pubmed-article:18589832 | pubmed:author | pubmed-author:HeBingB | lld:pubmed |
| pubmed-article:18589832 | pubmed:author | pubmed-author:ZhaoJuanJ | lld:pubmed |
| pubmed-article:18589832 | pubmed:author | pubmed-author:JiangHanminH | lld:pubmed |
| pubmed-article:18589832 | pubmed:author | pubmed-author:ChaiXinjunX | lld:pubmed |
| pubmed-article:18589832 | pubmed:author | pubmed-author:YuXindaX | lld:pubmed |
| pubmed-article:18589832 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18589832 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:18589832 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18589832 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18589832 | pubmed:pagination | 509-14 | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:meshHeading | pubmed-meshheading:18589832... | lld:pubmed |
| pubmed-article:18589832 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18589832 | pubmed:articleTitle | [Expression, purification and renaturation of proNGF in Escherichia coli]. | lld:pubmed |
| pubmed-article:18589832 | pubmed:affiliation | College of Life Sciences, Nankai University, Tianjin 300071, China. | lld:pubmed |
| pubmed-article:18589832 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18589832 | pubmed:publicationType | English Abstract | lld:pubmed |
| pubmed-article:18589832 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
