| pubmed-article:18585252 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18585252 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:18585252 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:18585252 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:18585252 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:18585252 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18585252 | pubmed:dateCreated | 2008-6-30 | lld:pubmed |
| pubmed-article:18585252 | pubmed:abstractText | By introducing heterocyclic compound to immunoassay system as an electrochemical substrate for the fist time, a new voltammetric enzyme-linked immunoassay system of 3-hydroxyl-2-aminopyridine (HAP)-H(2)O(2)-horseradish peroxidase (HRP) has been developed. HAP was oxidized with H(2)O(2) catalyzed by HRP, and the resulting electroactive product produced a sensitive voltammetric peak at potential of -0.36 V (vs. SCE) in Britton-Robinson (BR) buffer solution. The process of the enzyme-catalyzed reaction and the electro-reduction of the product have been investigated in detail. The linear range for detection of free HRP was from 4.0x10(-13) to 1.0x10(-9) g/mL with a detection limit of 1.2x10(-13) g/mL. The new system has been successfully applied for the assay of alpha-fetoprotein (alphaFP) in human serum ranging from 0.1 to 200 ng/mL with a detection limit of 0.1 ng/mL, which was 10 times lower than that of traditional spectrophotometric enzyme-linked immunosorbent assay (ELISA) method. HAP-H(2)O(2)-HRP voltammetric enzyme-linked immunoassay showed a promising alternative approach in the detection of alphaFP in clinical diagnosis. | lld:pubmed |
| pubmed-article:18585252 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18585252 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18585252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18585252 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18585252 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:18585252 | pubmed:issn | 1873-3573 | lld:pubmed |
| pubmed-article:18585252 | pubmed:author | pubmed-author:ZhangShusheng... | lld:pubmed |
| pubmed-article:18585252 | pubmed:author | pubmed-author:ZouJinJ | lld:pubmed |
| pubmed-article:18585252 | pubmed:author | pubmed-author:YuFengliF | lld:pubmed |
| pubmed-article:18585252 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18585252 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:18585252 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:18585252 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18585252 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18585252 | pubmed:pagination | 122-7 | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:meshHeading | pubmed-meshheading:18585252... | lld:pubmed |
| pubmed-article:18585252 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18585252 | pubmed:articleTitle | Investigation of voltammetric enzyme-linked immunoassay based on a new system of HAP-H2O2-HRP. | lld:pubmed |
| pubmed-article:18585252 | pubmed:affiliation | Key Laboratory of Eco-chemical Engineering, Ministry of Education; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. shushzhang@126.com | lld:pubmed |
| pubmed-article:18585252 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18585252 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
