| pubmed-article:18579134 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0335038 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0003374 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0311727 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0032150 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:18579134 | lifeskim:mentions | umls-concept:C0205132 | lld:lifeskim |
| pubmed-article:18579134 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18579134 | pubmed:dateCreated | 2008-8-11 | lld:pubmed |
| pubmed-article:18579134 | pubmed:abstractText | High-throughput screening (HTS) of small-molecule libraries against pharmacological targets is a key strategy of contemporary drug discovery. This study reports a simple, robust, and cell-based luminescent method for assaying antimalarial drugs. Using transfection technology, we generated a stable Plasmodium falciparum line with high levels of firefly luciferase expression. A luciferase assay based on this parasite line was optimized in a 96-well plate format and used to compare with the standard [(3)H] hypoxanthine radioisotope method. The 50% inhibitory concentrations (IC(50)s) of chloroquine, artesunate, artemether, dihydroartemisinin and curcumin obtained by these two methods were not significantly different (P>0.05, ANOVA). In addition, this assay could be performed conveniently with a luminescence plate reader using unsynchronized stages within as early as 12h. Furthermore, the luciferase assay is robust with a Z' score of 0.77-0.92, which suggests the feasibility for further miniaturization and automation. | lld:pubmed |
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| pubmed-article:18579134 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18579134 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18579134 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18579134 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18579134 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18579134 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18579134 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18579134 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18579134 | pubmed:issn | 1090-2449 | lld:pubmed |
| pubmed-article:18579134 | pubmed:author | pubmed-author:LiQianjunQ | lld:pubmed |
| pubmed-article:18579134 | pubmed:author | pubmed-author:WangJiaxuJ | lld:pubmed |
| pubmed-article:18579134 | pubmed:author | pubmed-author:CuiLongL | lld:pubmed |
| pubmed-article:18579134 | pubmed:author | pubmed-author:CuiLiwangL | lld:pubmed |
| pubmed-article:18579134 | pubmed:author | pubmed-author:MiaoJunJ | lld:pubmed |
| pubmed-article:18579134 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18579134 | pubmed:volume | 120 | lld:pubmed |
| pubmed-article:18579134 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18579134 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18579134 | pubmed:pagination | 80-7 | lld:pubmed |
| pubmed-article:18579134 | pubmed:dateRevised | 2010-12-3 | lld:pubmed |
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| pubmed-article:18579134 | pubmed:year | 2008 | lld:pubmed |
