| pubmed-article:18571492 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18571492 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:18571492 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:18571492 | lifeskim:mentions | umls-concept:C0019878 | lld:lifeskim |
| pubmed-article:18571492 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:18571492 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:18571492 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18571492 | pubmed:dateCreated | 2008-7-30 | lld:pubmed |
| pubmed-article:18571492 | pubmed:abstractText | Homocysteine (Hcy) is incorporated into protein via a reaction of the thioester Hcy-thiolactone with epsilon-amino group of a protein lysine residue. This reaction leads to impairment and alteration of protein's function and has been implicated in atherothrombotic disease. However, the data regarding N-linked Hcy content in proteins are limited, mostly due to a lack of facile assays. Here I describe a new sensitive assay for the determination of protein N-linked Hcy and demonstrate its utility for individual proteins and biological fluids. N-linked Hcy is liberated from proteins by acid hydrolysis and converted to Hcy-thiolactone, which is then purified and quantified by high-performance liquid chromatography on a cation exchange column. The quantification is by fluorescence after postcolumn derivatization with o-phthaldialdehyde. Using this assay, the levels of N-linked Hcy in individual pure proteins were found to vary from as high as 0.470-0.515 mol/mol protein for human and equine ferritins to as low as 0.00006 mol/mol protein for chicken lysozyme. Hemoglobins from a variety of species contained more N-linked Hcy than did corresponding albumins (0.0127-0.0828 vs. 0.0027-0.0086 mol/mol). Normal human plasma and milk were found to contain submicromolar concentrations of protein N-linked Hcy, whereas cow milk and whey contained micromolar concentrations of protein N-linked Hcy. | lld:pubmed |
| pubmed-article:18571492 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18571492 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18571492 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18571492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18571492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18571492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18571492 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18571492 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18571492 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:18571492 | pubmed:issn | 1096-0309 | lld:pubmed |
| pubmed-article:18571492 | pubmed:author | pubmed-author:JakubowskiHie... | lld:pubmed |
| pubmed-article:18571492 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18571492 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:18571492 | pubmed:volume | 380 | lld:pubmed |
| pubmed-article:18571492 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18571492 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18571492 | pubmed:pagination | 257-61 | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:meshHeading | pubmed-meshheading:18571492... | lld:pubmed |
| pubmed-article:18571492 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18571492 | pubmed:articleTitle | New method for the determination of protein N-linked homocysteine. | lld:pubmed |
| pubmed-article:18571492 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA. jakubows@umdnj.edu | lld:pubmed |
| pubmed-article:18571492 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18571492 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18571492 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18571492 | lld:pubmed |
