Linked Life Data

18560672

Source:http://linkedlifedata.com/resource/pubmed/id/18560672

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SubjectPredicateObjectContext
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pubmed-article:18560672pubmed:issue6lld:pubmed
pubmed-article:18560672pubmed:dateCreated2008-7-14lld:pubmed
pubmed-article:18560672pubmed:abstractTextApolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII SstI polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; >200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; <200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and DiaSys Diagnostic System International kit, respectively. A 428-bp sequence of apo-CIII gene was amplified using oligonucleotide primers 5' GGT GAC CGA TGG CTT CAG TTC CCT GA 3' and 5' CAG AAG GTG GAT AGA GCG CTG GCC T 3'. The PCR products were digested with a restriction endonuclease SstI. Rare G allele was highly prevalent in our study population (0.416) compared to Caucasians (0.00-0.11). G allele was almost two times more prevalent in the HTG group compared to the NTG group (P < 0.001). The genotype distribution was consistent with the Hardy-Weinberg equilibrium. There was a significant association between rare G allele and HTG in Japanese individuals living in Southern Brazil as indicated by one-way ANOVA, P < 0.05.lld:pubmed
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pubmed-article:18560672pubmed:year2008lld:pubmed
pubmed-article:18560672pubmed:articleTitleApolipoprotein CIII polymorphism and triglyceride levels of a Japanese population living in Southern Brazil.lld:pubmed
pubmed-article:18560672pubmed:affiliationDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.lld:pubmed
pubmed-article:18560672pubmed:publicationTypeJournal Articlelld:pubmed
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