| pubmed-article:18549648 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0003315 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C0039115 | lld:lifeskim |
| pubmed-article:18549648 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:18549648 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18549648 | pubmed:dateCreated | 2008-6-13 | lld:pubmed |
| pubmed-article:18549648 | pubmed:abstractText | This study was aimed to investigate the effect of artificial antigen-presenting cells (aAPCs) on inducing activation and proliferation of specific T-lymphocytes through stimulating biological function of dendritic cells with aAPCs in vitro. The specific antigen of chronic myeloid leukemia CML-28 was screened as objective antigen peptide by using magnetic microbeads as vector; the CML-28 epitope sequence (Vltfaldsv) was obtained by antigen epitope prediction software; this epitope was coupled with MHC molecule and used as first signal molecule, the B7-1 molecule was used as second signal molecule; these 2 molecules simultaneously were loaded onto surface of magnetic microbeads so as to contract aAPC complex. The bone marrow mononuclear cells were derived from HLA-A2(+) healthy bone marrow donors, CD8(+) T lymphocytes were screened and co-cultured with aAPC complex. During culture the 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) was added and proliferation of T-lymphocytes was detected by CPSE and proliferation level of specific T lymphocytes was detected by flow cytometry. The results showed that the proliferation level of CML-28 specific T lymphocytes obviously increased in experimental group, average level was 17.34 +/- 0.65%, while average level in control was 2.25 +/- 0.43%, there was significant difference between them (p < 0.01). It is concluded that the aAPC complex can mimic human APCs in vitro, and stimulate activation and proliferation of CD8(+) specific T lymphocytes. | lld:pubmed |
| pubmed-article:18549648 | pubmed:language | chi | lld:pubmed |
| pubmed-article:18549648 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18549648 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18549648 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18549648 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18549648 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18549648 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:18549648 | pubmed:issn | 1009-2137 | lld:pubmed |
| pubmed-article:18549648 | pubmed:author | pubmed-author:HuangWeiW | lld:pubmed |
| pubmed-article:18549648 | pubmed:author | pubmed-author:LiuWen-LiWL | lld:pubmed |
| pubmed-article:18549648 | pubmed:author | pubmed-author:ZhouJian-Feng... | lld:pubmed |
| pubmed-article:18549648 | pubmed:author | pubmed-author:GengZheZ | lld:pubmed |
| pubmed-article:18549648 | pubmed:author | pubmed-author:MaoHan-WenHW | lld:pubmed |
| pubmed-article:18549648 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18549648 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:18549648 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18549648 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18549648 | pubmed:pagination | 655-8 | lld:pubmed |
| pubmed-article:18549648 | pubmed:meshHeading | pubmed-meshheading:18549648... | lld:pubmed |
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| pubmed-article:18549648 | pubmed:meshHeading | pubmed-meshheading:18549648... | lld:pubmed |
| pubmed-article:18549648 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18549648 | pubmed:articleTitle | [Activation of specific T lymphocyte induced by artificial antigen presenting cell complex in vitro]. | lld:pubmed |
| pubmed-article:18549648 | pubmed:affiliation | Deparment of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. MHW555@21CN.COM | lld:pubmed |
| pubmed-article:18549648 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18549648 | pubmed:publicationType | English Abstract | lld:pubmed |
| pubmed-article:18549648 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
