| pubmed-article:185408 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:185408 | lifeskim:mentions | umls-concept:C0015767 | lld:lifeskim |
| pubmed-article:185408 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:185408 | lifeskim:mentions | umls-concept:C0025723 | lld:lifeskim |
| pubmed-article:185408 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:185408 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:185408 | pubmed:dateCreated | 1976-12-23 | lld:pubmed |
| pubmed-article:185408 | pubmed:abstractText | The high-molecular-weight subunit RNA of feline leukemia virus (Rickard strain) (FeLV-R) was analyzed for the presence of methyl groups. After purification of native 50-60S FeLV-R RNA on nondenaturing aqueous sucrose density gradients. FeLV-R 28S subunit RNA, doubly labeled with [14C]uridine and [methyl-3H]methionine, was isolated by centrifugation through denaturing sucrose density gradients in dimethyl sulfoxide. As calculated from their respective 3H/14C ratios. FeLV-R 28S RNA was methylated to the same degree as host cell poly(A)+ mRNA. When the 28S FeLV-R RNA was hydrolyzed to completion with RNase T2 or alkali, all of the methyl-3H chromatographed with mononucleotides on Pellionex-WAX, a weak anion exchanger. The methyl-labeled material co-chromatographed with 6-methyladenosine if the mononucleotide fraction obtained by Pellionex-WAX chromatography was hydrolyzed to nucleosides by bacterial alkaline phosphatase or with 6-methyladenine if purine bases were released from the mononucleotides by acid hydrolysis. In another experiment in which FeLV-R 28S RNA uniformly labeled with 32P was hydrolyzed and then analyzed by Pellionex-WAX chromatography, all of the 32P label again co-chromatographed with mononucleotides. Thus FeLV-R 28S RNA does not appear to contain a 5' structure, either methylated or nonmethylated similar to those recently reported for cellular and some animal virus mRNA's. | lld:pubmed |
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| pubmed-article:185408 | pubmed:language | eng | lld:pubmed |
| pubmed-article:185408 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:185408 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:185408 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:185408 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:185408 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:185408 | pubmed:author | pubmed-author:VelicerL FLF | lld:pubmed |
| pubmed-article:185408 | pubmed:author | pubmed-author:RottmanF MFM | lld:pubmed |
| pubmed-article:185408 | pubmed:author | pubmed-author:BrianD ADA | lld:pubmed |
| pubmed-article:185408 | pubmed:author | pubmed-author:ThomasonA RAR | lld:pubmed |
| pubmed-article:185408 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:185408 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:185408 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:185408 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:185408 | pubmed:pagination | 123-32 | lld:pubmed |
| pubmed-article:185408 | pubmed:dateRevised | 2010-9-1 | lld:pubmed |
| pubmed-article:185408 | pubmed:meshHeading | pubmed-meshheading:185408-M... | lld:pubmed |
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| pubmed-article:185408 | pubmed:meshHeading | pubmed-meshheading:185408-L... | lld:pubmed |
| pubmed-article:185408 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:185408 | pubmed:articleTitle | Methylation of high-molecular-weight subunit RNA of feline leukemia virus. | lld:pubmed |
| pubmed-article:185408 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:185408 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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