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pubmed-article:1851669 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:1851669 | pubmed:dateCreated | 1991-6-27 | lld:pubmed |
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pubmed-article:1851669 | pubmed:abstractText | We describe the cDNA encoding the SV40 transcriptional enhancer factor 1 (TEF-1) and show that its translation initiates exclusively at an AUU codon in vivo. Cloned TEF-1, which is unrelated to other known transcription factors, specifically binds the SV40 GT-IIC and Sph enhansons. Cloned TEF-1 does not activate these enhansons in lymphoid MPC11 cells where they are known to be inactive, but represses the endogenous HeLa TEF-1 activity in vivo and in vitro. Repression is also observed with chimeras where the DNA-binding domain of the GAL4 activator replaces that of TEF-1, showing that repression results from interference/squelching. Such chimeras stimulate transcription in HeLa, but not in MPC11, cells in vivo and in HeLa cell extracts in vitro. However, high concentrations result in self-interference/squelching. These results strongly suggest that the trans-activation function of TEF-1 is mediated by a highly limiting, possible cell-specific, titratable transcriptional intermediary factor(s). | lld:pubmed |
pubmed-article:1851669 | pubmed:language | eng | lld:pubmed |
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pubmed-article:1851669 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1851669 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1851669 | pubmed:month | May | lld:pubmed |
pubmed-article:1851669 | pubmed:issn | 0092-8674 | lld:pubmed |
pubmed-article:1851669 | pubmed:author | pubmed-author:ChambonPP | lld:pubmed |
pubmed-article:1851669 | pubmed:author | pubmed-author:GarnierJ MJM | lld:pubmed |
pubmed-article:1851669 | pubmed:author | pubmed-author:DavidsonII | lld:pubmed |
pubmed-article:1851669 | pubmed:author | pubmed-author:MatthesHH | lld:pubmed |
pubmed-article:1851669 | pubmed:author | pubmed-author:XiaoJ HJH | lld:pubmed |
pubmed-article:1851669 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1851669 | pubmed:day | 17 | lld:pubmed |
pubmed-article:1851669 | pubmed:volume | 65 | lld:pubmed |
pubmed-article:1851669 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1851669 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1851669 | pubmed:pagination | 551-68 | lld:pubmed |
pubmed-article:1851669 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:1851669 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:1851669 | pubmed:articleTitle | Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. | lld:pubmed |
pubmed-article:1851669 | pubmed:affiliation | Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Génie Génétique et de Biologie, Faculté de Médecine, Strasbourg, France. | lld:pubmed |
pubmed-article:1851669 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1851669 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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