| pubmed-article:1851071 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0025664 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0023401 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0031453 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C2825032 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0574032 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C0597297 | lld:lifeskim |
| pubmed-article:1851071 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:1851071 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1851071 | pubmed:dateCreated | 1991-6-13 | lld:pubmed |
| pubmed-article:1851071 | pubmed:abstractText | 1. The aims of this study were twofold: (i) to investigate the ability of a recently described [2H5]phenylalanine method for quantifying whole-body protein turnover during acute physiological perturbation; (ii) to determine specifically whether the previously observed increase in protein synthesis on insulin withdrawal in insulin-dependent (type 1) diabetic patients seen when employing the [13C]leucine technique could be corroborated by using [2H5]phenylalanine. 2. Whole-body protein turnover was measured by both the [2H5]phenylalanine and [13C]leucine primed continuous infusion methods applied simultaneously to six type I post-absorptive diabetic patients during insulin withdrawal and infusion. 3. Values were determined by the [13C]leucine method by measuring either [13C]leucine (primary pool) or alpha-[13C] ketoisocaproic acid (reciprocal pool) enrichment in plasma. 4. Values of whole-body protein breakdown during insulin withdrawal derived from the [2H5]phenylalanine and primary and reciprocal pool [13C]leucine models respectively were 3.54 +/- 0.43, 3.85 +/- 0.41 and 4.62 +/- 0.44 g day-1 kg-1 (means +/- SD). Insulin infusion resulted in a significant reduction (P less than 0.02) to 3.07 +/- 0.34, 3.05 +/- 0.26 and 3.82 +/- 0.4 g day-1 kg-1, respectively. Synthesis values fell significantly but by a smaller amount than breakdown, resulting in increased (P less than 0.05) net protein deposition, regardless of the model used. 5. These data demonstrate that the [2H5]phenylalanine and [13C]leucine methods generate similar results both in absolute and relative terms in response to short-term insulin infusion. 6. The confirmation of increased whole-body protein synthesis during insulin withdrawal by two independent methods supports the validity of this observation. | lld:pubmed |
| pubmed-article:1851071 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1851071 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1851071 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1851071 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1851071 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1851071 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1851071 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1851071 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1851071 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1851071 | pubmed:issn | 0143-5221 | lld:pubmed |
| pubmed-article:1851071 | pubmed:author | pubmed-author:HallidayDD | lld:pubmed |
| pubmed-article:1851071 | pubmed:author | pubmed-author:ThompsonG NGN | lld:pubmed |
| pubmed-article:1851071 | pubmed:author | pubmed-author:PacyP JPJ | lld:pubmed |
| pubmed-article:1851071 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1851071 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:1851071 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1851071 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1851071 | pubmed:pagination | 345-52 | lld:pubmed |
| pubmed-article:1851071 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:meshHeading | pubmed-meshheading:1851071-... | lld:pubmed |
| pubmed-article:1851071 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1851071 | pubmed:articleTitle | Measurement of whole-body protein turnover in insulin-dependent (type 1) diabetic patients during insulin withdrawal and infusion: comparison of [13C]leucine and [2H5]phenylalanine methodologies. | lld:pubmed |
| pubmed-article:1851071 | pubmed:affiliation | Nutrition Research Group, Clinical Research Centre, Harrow, Middlesex, U.K. | lld:pubmed |
| pubmed-article:1851071 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1851071 | pubmed:publicationType | Clinical Trial | lld:pubmed |
| pubmed-article:1851071 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1851071 | pubmed:publicationType | Controlled Clinical Trial | lld:pubmed |
