| pubmed-article:1850970 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0949782 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C1564139 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C1533693 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:1850970 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:1850970 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1850970 | pubmed:dateCreated | 1991-5-31 | lld:pubmed |
| pubmed-article:1850970 | pubmed:abstractText | Using the techniques of gap misrepair mutagenesis and site-directed mutagenesis, we have generated two novel quinolone resistance mutations of the Escherichia coli DNA gyrase A protein. DNA sequencing showed these mutations to be Ser-83----Ala and Gln-106----Arg. The mutant proteins were overproduced and purified, and their enzymatic properties were analyzed and compared with those of the wild-type enzyme. With ciprofloxacin and other quinolones, the inhibition of DNA supercoiling, relaxation, and decatenation and the induction of DNA cleavage were investigated for both wild-type and mutant enzymes. In each assay, the mutant enzymes were found to require approximately 10 times more drug to inhibit the reaction or induce cleavage than was the wild-type enzyme. However, the Ca2(+)-directed DNA cleavage reaction was indistinguishable for wild-type and mutant gyrases. We discuss models for the gyrase-mediated bactericidal effects of quinolone drugs. | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1850970 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1850970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850970 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1850970 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1850970 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:1850970 | pubmed:author | pubmed-author:HallettPP | lld:pubmed |
| pubmed-article:1850970 | pubmed:author | pubmed-author:MaxwellAA | lld:pubmed |
| pubmed-article:1850970 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1850970 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:1850970 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1850970 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1850970 | pubmed:pagination | 335-40 | lld:pubmed |
| pubmed-article:1850970 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:meshHeading | pubmed-meshheading:1850970-... | lld:pubmed |
| pubmed-article:1850970 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1850970 | pubmed:articleTitle | Novel quinolone resistance mutations of the Escherichia coli DNA gyrase A protein: enzymatic analysis of the mutant proteins. | lld:pubmed |
| pubmed-article:1850970 | pubmed:affiliation | Department of Biochemistry, University of Leicester, United Kingdom. | lld:pubmed |
| pubmed-article:1850970 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1850970 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:946614 | entrezgene:pubmed | pubmed-article:1850970 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850970 | lld:pubmed |
