| pubmed-article:18480090 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C0872315 | lld:lifeskim |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C0302350 | lld:lifeskim |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C1880355 | lld:lifeskim |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C2698650 | lld:lifeskim |
| pubmed-article:18480090 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:18480090 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:18480090 | pubmed:dateCreated | 2008-7-15 | lld:pubmed |
| pubmed-article:18480090 | pubmed:abstractText | Using a comprehensive set of discovery and optimization tools, antibodies were produced with the ability to neutralize SARS coronavirus (SARS-CoV) infection in Vero E6 cells and in animal models. These anti-SARS antibodies were discovered using a novel DNA display method, which can identify new antibodies within days. Once neutralizing antibodies were identified, a comprehensive and effective means of converting the mouse sequences to human frameworks was accomplished using HuFR (human framework reassembly) technology. The best variant (61G4) from this screen showed a 3.5-4-fold improvement in neutralization of SARS-CoV infection in vitro. Finally, using a complete site-saturation mutagenesis methodology focused on the CDR (complementarity determining regions), a single point mutation (51E7) was identified that improved the 80% plaque reduction neutralization of the virus by greater than 8-fold. These discovery and evolution strategies can be applied to any emerging pathogen or toxin where a causative agent is known. | lld:pubmed |
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| pubmed-article:18480090 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18480090 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18480090 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18480090 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18480090 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18480090 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:18480090 | pubmed:issn | 1741-0126 | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:KellerMM | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:WongKK | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:WoodnuttGG | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:RogersJJ | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:OOIS KSK | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:LewisL MLM | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:SchröderOO | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:HollandT FTF | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:SchoeppR JRJ | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:KimmelB EBE | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:UDAKK | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:LiJ QJQ | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:SatoA KAK | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:TozerE CEC | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:ClementsT LTL | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:DirmeierR PRP | lld:pubmed |
| pubmed-article:18480090 | pubmed:author | pubmed-author:FreyG JGJ | lld:pubmed |
| pubmed-article:18480090 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18480090 | pubmed:volume | 21 | lld:pubmed |
| pubmed-article:18480090 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18480090 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18480090 | pubmed:pagination | 495-505 | lld:pubmed |
| pubmed-article:18480090 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:18480090 | pubmed:meshHeading | pubmed-meshheading:18480090... | lld:pubmed |
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| pubmed-article:18480090 | pubmed:meshHeading | pubmed-meshheading:18480090... | lld:pubmed |
| pubmed-article:18480090 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18480090 | pubmed:articleTitle | Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases. | lld:pubmed |
| pubmed-article:18480090 | pubmed:affiliation | Verenium Corporation, 4955 Directors Place, San Diego, CA 92121, USA. | lld:pubmed |
| pubmed-article:18480090 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18480090 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
