| pubmed-article:1847370 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0019168 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0023828 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0949374 | lld:lifeskim |
| pubmed-article:1847370 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1847370 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1847370 | pubmed:dateCreated | 1991-3-27 | lld:pubmed |
| pubmed-article:1847370 | pubmed:abstractText | We established a simple and efficient method for gene transfer in vitro (to cultured cells) and in vivo (to an adult organ) using liposomes. Plasmid DNA and proteins were efficiently co-encapsulated in liposomes by agitation and sonication, and were co-introduced into cells by hemagglutinating virus of Japan (HVJ)-mediated membrane fusion. Introduction of the Escherichia coli beta-galactosidase gene with non-histone chromosomal protein high mobility group 1 (HMG1) into LLCMK2 cells resulted in about 3 times higher beta-galactosidase activity than that on introduction of the gene alone. Two days after injection of HVJ-liposomes containing the beta-galactosidase gene and HMG1 under the perisplanchnic membrane of adult rat liver, hepatic cells near the injection site were found by 5-bromo-4-chloro-3-indolyl beta-D-galactoside staining to have beta-galactosidase activity. After similar injection of HVJ-liposomes containing the hepatitis B virus surface antigen (HBsAg) gene and HMG1, HBsAg was detected in the serum for 9 days with a maximum of 25-45 ng/ml on day 2 after the injection. | lld:pubmed |
| pubmed-article:1847370 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1847370 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1847370 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1847370 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1847370 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1847370 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1847370 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1847370 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1847370 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1847370 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1847370 | pubmed:author | pubmed-author:KatoKK | lld:pubmed |
| pubmed-article:1847370 | pubmed:author | pubmed-author:OkadaYY | lld:pubmed |
| pubmed-article:1847370 | pubmed:author | pubmed-author:NakanishiMM | lld:pubmed |
| pubmed-article:1847370 | pubmed:author | pubmed-author:UchidaTT | lld:pubmed |
| pubmed-article:1847370 | pubmed:author | pubmed-author:KanedaYY | lld:pubmed |
| pubmed-article:1847370 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1847370 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1847370 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:1847370 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1847370 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1847370 | pubmed:pagination | 3361-4 | lld:pubmed |
| pubmed-article:1847370 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1847370 | pubmed:meshHeading | pubmed-meshheading:1847370-... | lld:pubmed |
| pubmed-article:1847370 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1847370 | pubmed:articleTitle | Expression of hepatitis B virus surface antigen in adult rat liver. Co-introduction of DNA and nuclear protein by a simplified liposome method. | lld:pubmed |
| pubmed-article:1847370 | pubmed:affiliation | Institute for Molecular and Cellular Biology, Osaka University, Japan. | lld:pubmed |
| pubmed-article:1847370 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1847370 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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