| pubmed-article:18440975 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0212694 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0028778 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0332206 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C1707511 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0597094 | lld:lifeskim |
| pubmed-article:18440975 | lifeskim:mentions | umls-concept:C0444930 | lld:lifeskim |
| pubmed-article:18440975 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:18440975 | pubmed:dateCreated | 2008-6-9 | lld:pubmed |
| pubmed-article:18440975 | pubmed:abstractText | Previous work showed that, in the presence of DNA-dependent protein kinase (DNA-PK), Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5'-->3' exonucleolytic resection of double-stranded DNA. This resection required a 5'-phosphate, but did not require ATP, and was accompanied by endonucleolytic cleavage of the resulting 3' overhang. In the presence of DNA-PK, Artemis-mediated trimming was more limited, was ATP-dependent and did not require a 5'-phosphate. For a blunt end with either a 3'-phosphoglycolate or 3'-hydroxyl terminus, endonucleolytic trimming of 2-4 nucleotides from the 3'-terminal strand was accompanied by trimming of 6 nt from the 5'-terminal strand. The results suggest that autophosphorylated DNA-PK suppresses the exonuclease activity of Artemis toward blunt-ended DNA, and promotes slow and limited endonucleolytic trimming of the 5'-terminal strand, resulting in short 3' overhangs that are trimmed endonucleolytically. Thus, Artemis and DNA-PK can convert terminally blocked DNA ends of diverse geometry and chemical structure to a form suitable for polymerase-mediated patching and ligation, with minimal loss of terminal sequence. Such processing could account for the very small deletions often found at DNA double-strand break repair sites. | lld:pubmed |
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| pubmed-article:18440975 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18440975 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18440975 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:18440975 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18440975 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:18440975 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:ZhouTongT | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:PovirkLawrenc... | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:ValerieKristo... | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:YannoneSteven... | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:ZhouRui-ZheRZ | lld:pubmed |
| pubmed-article:18440975 | pubmed:author | pubmed-author:KhanImran SIS | lld:pubmed |
| pubmed-article:18440975 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18440975 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:18440975 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18440975 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18440975 | pubmed:pagination | 3354-65 | lld:pubmed |
| pubmed-article:18440975 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:18440975 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18440975 | pubmed:articleTitle | Coordinate 5' and 3' endonucleolytic trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase. | lld:pubmed |
| pubmed-article:18440975 | pubmed:affiliation | Life Sciences Division, Department of Molecular Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. | lld:pubmed |
| pubmed-article:18440975 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18440975 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:18440975 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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