| pubmed-article:1840587 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C0995594 | lld:lifeskim |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C0004641 | lld:lifeskim |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1840587 | lifeskim:mentions | umls-concept:C0125581 | lld:lifeskim |
| pubmed-article:1840587 | pubmed:issue | 23 | lld:pubmed |
| pubmed-article:1840587 | pubmed:dateCreated | 1991-12-26 | lld:pubmed |
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| pubmed-article:1840587 | pubmed:abstractText | Leucocin A-UAL 187 is a bacteriocin produced by Leuconostoc gelidum UAL 187, a lactic acid bacterium isolated from vacuum-packaged meat. The bacteriocin was purified by ammonium sulfate or acid (pH 2.5) precipitation, hydrophobic interaction chromatography, gel filtration, and reversed-phase high-performance liquid chromatography with a yield of 58% of the original activity. Leucocin A is stable at low pH and heat resistant, and the activity of the pure form is enhanced by the addition of bovine serum albumin. It is inactivated by a range of proteolytic enzymes. The molecular weight was determined by mass spectrometry to be 3,930.3 +/- 0.4. Leucocin A-UAL 187 contains 37 amino acids with a calculated molecular weight of 3,932.3. A mixed oligonucleotide (24-mer) homologous to the sequence of the already known N terminus of the bacteriocin hybridized to a 2.9-kb HpaII fragment of a 7.6-MDa plasmid from the producer strain. The fragment was cloned into pUC118 and then subcloned into a lactococcal shuttle vector, pNZ19. DNA sequencing revealed an operon consisting of a putative upstream promoter, a downstream terminator, and two open reading frames flanked by a putative upstream promoter and a downstream terminator. The first open reading frame downstream of the promoter contains 61 amino acids and is identified as the leucocin structural gene, consisting of a 37-amino-acid bacteriocin and a 24-residue N-terminal extension. No phenotypic expression of the bacteriocin was evident in several lactic acid bacteria that were electrotransformed with pNZ19 containing the 2.9-kb cloned fragment of the leucocin A plasmid. | lld:pubmed |
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| pubmed-article:1840587 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1840587 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1840587 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1840587 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1840587 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1840587 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:HastingsJ WJW | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:VederasJ CJC | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:JohnsonKK | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:StilesM EME | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:RoyK LKL | lld:pubmed |
| pubmed-article:1840587 | pubmed:author | pubmed-author:SailerMM | lld:pubmed |
| pubmed-article:1840587 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1840587 | pubmed:volume | 173 | lld:pubmed |
| pubmed-article:1840587 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1840587 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1840587 | pubmed:pagination | 7491-500 | lld:pubmed |
| pubmed-article:1840587 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1840587 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1840587 | pubmed:articleTitle | Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. | lld:pubmed |
| pubmed-article:1840587 | pubmed:affiliation | Department of Food Science, University of Alberta, Edmonton, Canada. | lld:pubmed |
| pubmed-article:1840587 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1840587 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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