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pubmed-article:18379780pubmed:abstractTextThis study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medium for the sustainable growth of Mannheimia succiniciproducens, while no substitutable essential compounds were identified. The in silico predictions were verified by cultivating the cells on a CDM containing the six non-substitutable essential compounds, and it was further demonstrated by observing no cell growth on the CDM lacking any one of the non-substitutable essentials. An optimal CDM for the enhancement of cell growth and succinic acid production, as a target product, was formulated with a single-addition technique. The fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation using a complex medium. The optimal CDM also lowered the sum of the amounts of by-products (acetic, formic, and lactic acids) by 30%. The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available.lld:pubmed
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pubmed-article:18379780pubmed:authorpubmed-author:ChangHo NamHNlld:pubmed
pubmed-article:18379780pubmed:authorpubmed-author:LeeSang YupSYlld:pubmed
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pubmed-article:18379780pubmed:authorpubmed-author:KimTae YongTYlld:pubmed
pubmed-article:18379780pubmed:authorpubmed-author:ChoiSeong...lld:pubmed
pubmed-article:18379780pubmed:authorpubmed-author:SongHyohakHlld:pubmed
pubmed-article:18379780pubmed:authorpubmed-author:ChoiBo-Kyeong...lld:pubmed
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pubmed-article:18379780pubmed:volume79lld:pubmed
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pubmed-article:18379780pubmed:pagination263-72lld:pubmed
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pubmed-article:18379780pubmed:year2008lld:pubmed
pubmed-article:18379780pubmed:articleTitleDevelopment of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence.lld:pubmed
pubmed-article:18379780pubmed:affiliationDepartment of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.lld:pubmed
pubmed-article:18379780pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:18379780pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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