| pubmed-article:18373083 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0022262 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0038774 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0079240 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:18373083 | lifeskim:mentions | umls-concept:C0205344 | lld:lifeskim |
| pubmed-article:18373083 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18373083 | pubmed:dateCreated | 2010-11-30 | lld:pubmed |
| pubmed-article:18373083 | pubmed:abstractText | Absolute protein quantification has become an important challenge in modern bioanalytical chemistry. Among several approaches based on mass spectrometric techniques, inductively coupled plasma (ICP) as ionisation source provides element-selective and sensitive detection of heteroatoms, and thus, a potentially emerging tool in protein analysis. In this work we applied coupling of capillary liquid chromatography (?LC) and inductively coupled plasma-sector field mass spectrometry (ICP-SFMS) to the separation and determination of standard proteins. For quantification purposes, post-column isotope dilution of sulfur was applied and optimised for this type of hyphenated technique. Provided that the protein sequence is known (number of sulfur-containing amino acids, i.e. cysteines and methionines) the protein amount can then be directly calculated from the determined sulfur content in a certain protein fraction. In order to prove the reliability of the presented method, two different certified reference materials were analysed: CRM 393 (human apolipoprotein A-I) and CRM 486 (?-fetoprotein). For CRM 393 excellent agreement (37.0?±?1.4 ?mol L(-1)) was obtained with the certificate (37.7?±?1.8 ?mol L(-1)). However, the recovery rate for ?-fetoprotein in CRM 486 was found to be about 60% indicating incomplete elution of the protein during the chromatographic separation. | lld:pubmed |
| pubmed-article:18373083 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18373083 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18373083 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18373083 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18373083 | pubmed:month | May | lld:pubmed |
| pubmed-article:18373083 | pubmed:issn | 1618-2650 | lld:pubmed |
| pubmed-article:18373083 | pubmed:author | pubmed-author:KrügerRalfR | lld:pubmed |
| pubmed-article:18373083 | pubmed:author | pubmed-author:LeonhardPeter... | lld:pubmed |
| pubmed-article:18373083 | pubmed:author | pubmed-author:BettmerJörgJ | lld:pubmed |
| pubmed-article:18373083 | pubmed:author | pubmed-author:ZinnNicoN | lld:pubmed |
| pubmed-article:18373083 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18373083 | pubmed:volume | 391 | lld:pubmed |
| pubmed-article:18373083 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18373083 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18373083 | pubmed:pagination | 537-43 | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:meshHeading | pubmed-meshheading:18373083... | lld:pubmed |
| pubmed-article:18373083 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18373083 | pubmed:articleTitle | ?LC coupled to ICP-SFMS with post-column isotope dilution analysis of sulfur for absolute protein quantification. | lld:pubmed |
| pubmed-article:18373083 | pubmed:affiliation | Molecular Structure Analysis, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany. | lld:pubmed |
| pubmed-article:18373083 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18373083 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
