| pubmed-article:18316033 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18316033 | lifeskim:mentions | umls-concept:C0018026 | lld:lifeskim |
| pubmed-article:18316033 | lifeskim:mentions | umls-concept:C1101610 | lld:lifeskim |
| pubmed-article:18316033 | lifeskim:mentions | umls-concept:C1450054 | lld:lifeskim |
| pubmed-article:18316033 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:18316033 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18316033 | pubmed:dateCreated | 2008-4-15 | lld:pubmed |
| pubmed-article:18316033 | pubmed:abstractText | MicroRNAs are important posttranscriptional regulators of gene expression in animals and plants. A sensitive and specific detection method is urgently needed for intensive studies on differential expression and regulatory roles of microRNA. Here we present a simple and reliable method for the quantification of microRNA. The hybridization products of target microRNA with capture probe and gold nanoparticle probe are immobilized onto the surface of a streptavidin-coated microplate, and the signal is amplified by silver enhancement. Distribution of miR-122a/miR-128 in mouse brain and liver tissue was detected by this method, and synthetic miRNA122a was quantified. This method allowed a lower detect limit of 10 fM with a linear dynamic range from 10 pM to 10 fM and a high specificity to discriminate one single oligonucleotide mismatch of the target microRNA. | lld:pubmed |
| pubmed-article:18316033 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18316033 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18316033 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18316033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18316033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18316033 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18316033 | pubmed:month | May | lld:pubmed |
| pubmed-article:18316033 | pubmed:issn | 1096-0309 | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:RamS BSB | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:ZhengHaoH | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:MiaoLiL | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:XuShun-QingSQ | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:LiYuan-YuanYY | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:ChenTianT | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:LiXiao-BoXB | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:YangWen-JieWJ | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:ZhaoLi-FanLF | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:HeWen-LeiWL | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:GaoYu-QiuYQ | lld:pubmed |
| pubmed-article:18316033 | pubmed:author | pubmed-author:WanYan-JianYJ | lld:pubmed |
| pubmed-article:18316033 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18316033 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:18316033 | pubmed:volume | 376 | lld:pubmed |
| pubmed-article:18316033 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18316033 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18316033 | pubmed:pagination | 183-8 | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:meshHeading | pubmed-meshheading:18316033... | lld:pubmed |
| pubmed-article:18316033 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18316033 | pubmed:articleTitle | Quantification of microRNA by gold nanoparticle probes. | lld:pubmed |
| pubmed-article:18316033 | pubmed:affiliation | MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, wuhan 430030, China. | lld:pubmed |
| pubmed-article:18316033 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18316033 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
