| pubmed-article:18313997 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0023516 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:18313997 | lifeskim:mentions | umls-concept:C0049718 | lld:lifeskim |
| pubmed-article:18313997 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:18313997 | pubmed:dateCreated | 2008-3-17 | lld:pubmed |
| pubmed-article:18313997 | pubmed:abstractText | A HPLC method for determination of 6-thioguanine nucleotide in DNA was developed. Leukocyte DNA was isolated from peripheral blood, derivatized with chloroacetaldehyde and the formed etheno derivatives N(2),3-etheno 6-thioguanine (epsilon6TG), 1,N(6)-etheno adenine (epsilonA) and N(2),3-etheno guanine (epsilonG) were released from the DNA backbone by hydrolysis at pH 6.0 and 80 degrees C for 60 min. After extraction of epsilon6TG by immobilized metal ion affinity chromatography (IMAC) the sample was analysed by ion-pair reversed-phase HPLC with fluorescence detection. The limit of quantification was 9.0 nM and the intra- and interday precision ranged from 2.8 to 15.5%. In a small cohort of eight children with acute lymphoblastic leukaemia (ALL), a median of one 6-thioguanine base was found for each 3000 normal bases (range 1:2000-1:11000). | lld:pubmed |
| pubmed-article:18313997 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18313997 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18313997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18313997 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18313997 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:18313997 | pubmed:issn | 1570-0232 | lld:pubmed |
| pubmed-article:18313997 | pubmed:author | pubmed-author:HansenSteen... | lld:pubmed |
| pubmed-article:18313997 | pubmed:author | pubmed-author:SchmiegelowKj... | lld:pubmed |
| pubmed-article:18313997 | pubmed:author | pubmed-author:SideniusUlrik... | lld:pubmed |
| pubmed-article:18313997 | pubmed:author | pubmed-author:OlesenKaren-M... | lld:pubmed |
| pubmed-article:18313997 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18313997 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:18313997 | pubmed:volume | 864 | lld:pubmed |
| pubmed-article:18313997 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18313997 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18313997 | pubmed:pagination | 149-55 | lld:pubmed |
| pubmed-article:18313997 | pubmed:meshHeading | pubmed-meshheading:18313997... | lld:pubmed |
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| pubmed-article:18313997 | pubmed:meshHeading | pubmed-meshheading:18313997... | lld:pubmed |
| pubmed-article:18313997 | pubmed:meshHeading | pubmed-meshheading:18313997... | lld:pubmed |
| pubmed-article:18313997 | pubmed:meshHeading | pubmed-meshheading:18313997... | lld:pubmed |
| pubmed-article:18313997 | pubmed:meshHeading | pubmed-meshheading:18313997... | lld:pubmed |
| pubmed-article:18313997 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18313997 | pubmed:articleTitle | Determination of leukocyte DNA 6-thioguanine nucleotide levels by high-performance liquid chromatography with fluorescence detection. | lld:pubmed |
| pubmed-article:18313997 | pubmed:affiliation | Department of Pharmaceuticals and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. kmo@farma.ku.dk <kmo@farma.ku.dk> | lld:pubmed |
| pubmed-article:18313997 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18313997 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
