| pubmed-article:18305194 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C0025424 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C0600364 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C1708481 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:18305194 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:18305194 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18305194 | pubmed:dateCreated | 2008-3-24 | lld:pubmed |
| pubmed-article:18305194 | pubmed:abstractText | Mercury is a ubiquitous pollutant that when absorbed is extremely toxic to a wide variety of biochemical processes. Mercury (II) is a strong, "invisible" poison that is rapidly absorbed by tissues of the intestinal tract, kidneys, and liver upon ingestion. In this study, a novel fluorescence-based biosensor is presented that allows for the direct monitoring of the uptake and distribution of the metal under noninvasive in vivo conditions. With the introduction of a cysteine residue at position 205, located in close proximity to the chromophore, the green fluorescent protein (GFP) from Aequorea victoria was converted into a highly specific biosensor for this metal ion. The mutant protein exhibits a dramatic absorbance and fluorescence change upon mercuration at neutral pH. Absorbance and fluorescence properties with respect to the metal concentration exhibit sigmoidal binding behavior with a detection limit in the low nanomolar range. Time-resolved binding studies indicate rapid subsecond binding of the metal to the protein. The crystal structures obtained of mutant eGFP205C indicate a possible access route of the metal into the core of the protein. To our knowledge, this engineered protein is a first example of a biosensor that allows for noninvasive and real-time imaging of mercury uptake in a living cell. A major advantage is that its expression can be genetically controlled in many organisms to enable unprecedented studies of tissue specific mercury uptake. | lld:pubmed |
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| pubmed-article:18305194 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18305194 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18305194 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18305194 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18305194 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18305194 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18305194 | pubmed:issn | 1469-896X | lld:pubmed |
| pubmed-article:18305194 | pubmed:author | pubmed-author:SagermannMart... | lld:pubmed |
| pubmed-article:18305194 | pubmed:author | pubmed-author:FordPeter CPC | lld:pubmed |
| pubmed-article:18305194 | pubmed:author | pubmed-author:ChapleauRicha... | lld:pubmed |
| pubmed-article:18305194 | pubmed:author | pubmed-author:BlombergRebec... | lld:pubmed |
| pubmed-article:18305194 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18305194 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:18305194 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18305194 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18305194 | pubmed:pagination | 614-22 | lld:pubmed |
| pubmed-article:18305194 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
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| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
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| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:meshHeading | pubmed-meshheading:18305194... | lld:pubmed |
| pubmed-article:18305194 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18305194 | pubmed:articleTitle | Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake. | lld:pubmed |
| pubmed-article:18305194 | pubmed:affiliation | Department of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, California 93106-9510, USA. | lld:pubmed |
| pubmed-article:18305194 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18305194 | lld:pubmed |
