| pubmed-article:18302483 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1704939 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0018956 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0333873 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0279038 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1424622 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1538698 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1524108 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0815304 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:18302483 | lifeskim:mentions | umls-concept:C0444782 | lld:lifeskim |
| pubmed-article:18302483 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18302483 | pubmed:dateCreated | 2008-2-27 | lld:pubmed |
| pubmed-article:18302483 | pubmed:abstractText | Tumor radiotherapy with large-field irradiation results in an increase in apoptosis of the radiosensitive hematopoietic stem cells (CD34(+)). The aim of this study was to demonstrate the radioprotective potential of MDR1 overexpression in human CD34(+) cells using a lentiviral self-inactivating vector. Transduced human undifferentiated CD34(+) cells were irradiated with 0-8 Gy and held in liquid culture under myeloid-specific maturation conditions. After 12 days, MDR1 expression was determined by the rhodamine efflux assay. The proportion of MDR1-positive cells in cells from four human donors increased with increasing radiation dose (up to a 14-fold increase at 8 Gy). Determination of expression of myeloid-specific surface marker proteins revealed that myeloid differentiation was not affected by transduction and MDR1 overexpression. Irradiation after myeloid differentiation also led to an increase of MDR1-positive cells with escalating radiation doses (e.g. 12.5-16% from 0-8 Gy). Most importantly, fractionated irradiation (3 x 2 Gy; 24-h intervals) of MDR1-transduced CD34(+) cells resulted in an increase in MDR1-positive cells (e.g. 3-8% from 0-3 x 2 Gy). Our results clearly support a radioprotective effect of lentiviral MDR1 overexpression in human CD34(+) cells. Thus enhancing repopulation by surviving stem cells may increase the radiation tolerance of the hematopoietic system, which will contribute to widening the therapeutic index in radiotherapy. | lld:pubmed |
| pubmed-article:18302483 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18302483 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18302483 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18302483 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18302483 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18302483 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18302483 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18302483 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18302483 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:18302483 | pubmed:issn | 0033-7587 | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:LaufsStephani... | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:ZellerW... | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:FruehaufStefa... | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:WenzFrederikF | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:MaierPatrickP | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:FleckensteinK... | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:HerskindCarst... | lld:pubmed |
| pubmed-article:18302483 | pubmed:author | pubmed-author:SpierIsabelI | lld:pubmed |
| pubmed-article:18302483 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18302483 | pubmed:volume | 169 | lld:pubmed |
| pubmed-article:18302483 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18302483 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18302483 | pubmed:pagination | 301-10 | lld:pubmed |
| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
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| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
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| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
| pubmed-article:18302483 | pubmed:meshHeading | pubmed-meshheading:18302483... | lld:pubmed |
| pubmed-article:18302483 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18302483 | pubmed:articleTitle | MDR1 gene transfer using a lentiviral SIN vector confers radioprotection to human CD34+ hematopoietic progenitor cells. | lld:pubmed |
| pubmed-article:18302483 | pubmed:affiliation | Department of Radiation Oncology, Mannheim Medical Centre, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. patrick.maier@radonk.ma.uni-heidelberg.de | lld:pubmed |
| pubmed-article:18302483 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18302483 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
