| pubmed-article:1830127 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C0027923 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C0597295 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C0332287 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C1823614 | lld:lifeskim |
| pubmed-article:1830127 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:1830127 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:1830127 | pubmed:dateCreated | 1991-8-21 | lld:pubmed |
| pubmed-article:1830127 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1830127 | pubmed:abstractText | The cyt-20-1 mutant of Neurospora crassa is a temperature-sensitive, cytochrome b- and aa3-deficient strain that is severely deficient in both mitochondrial and cytosolic protein synthesis (R.A. Collins, H. Bertrand, R.J. LaPolla, and A.M. Lambowitz, Mol. Gen. Genet. 177:73-84, 1979). We cloned the cyt-20+ gene by complementation of the cyt-20-1 mutation and found that it contains a 1,093-amino-acid open reading frame (ORF) that encodes both the cytosolic and mitochondrial valyl-tRNA synthetases (vaIRSs). A second mutation, un-3, which is allelic with cyt-20-1, also results in temperature-sensitive growth, but not in gross deficiencies in cytochromes b and aa3 or protein synthesis. The un-3 mutant had also been reported to have pleiotropic defects in cellular transport process, resulting in resistance to amino acid analogs (M.S. Kappy and R.L. Metzenberg, J. Bacteriol. 94:1629-1637, 1967), but this resistance phenotype is separable from the temperature sensitivity in crosses and may result from a mutation in a different gene. The 1,093-amino-acid ORF encoding vaIRSs is the site of missense mutations resulting in temperature sensitivity in both cyt-20-1 and un-3 and is required for the transformation of both mutants. The opposite strand of the cyt-20 gene encodes an overlapping ORF of 532 amino acids, which may also be functional but is not required for transformation of either mutant. The cyt-20-1 mutation in the vaIRS ORF results in severe deficiencies of both mitochondrial and cytosolic vaIRS activities, whereas the un-3 mutation does not appear to result in a deficiency of these activities or of mitochondrial or cytosolic protein synthesis sufficient to account for its temperature-sensitive growth. The phenotype of the un-3 mutant raises the possibility that the vaIRS ORF has a second function in addition to protein synthesis. | lld:pubmed |
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| pubmed-article:1830127 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1830127 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1830127 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1830127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1830127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1830127 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1830127 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1830127 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:1830127 | pubmed:author | pubmed-author:LambowitzA... | lld:pubmed |
| pubmed-article:1830127 | pubmed:author | pubmed-author:TurckDD | lld:pubmed |
| pubmed-article:1830127 | pubmed:author | pubmed-author:KubelikA RAR | lld:pubmed |
| pubmed-article:1830127 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1830127 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:1830127 | pubmed:geneSymbol | leuRS | lld:pubmed |
| pubmed-article:1830127 | pubmed:geneSymbol | cyt-18 | lld:pubmed |
| pubmed-article:1830127 | pubmed:geneSymbol | cyt-20 | lld:pubmed |
| pubmed-article:1830127 | pubmed:geneSymbol | leu-5 | lld:pubmed |
| pubmed-article:1830127 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1830127 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1830127 | pubmed:pagination | 4022-35 | lld:pubmed |
| pubmed-article:1830127 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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| pubmed-article:1830127 | pubmed:meshHeading | pubmed-meshheading:1830127-... | lld:pubmed |
