| pubmed-article:18295717 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C0038975 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C0596290 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:18295717 | lifeskim:mentions | umls-concept:C0023043 | lld:lifeskim |
| pubmed-article:18295717 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18295717 | pubmed:dateCreated | 2008-2-25 | lld:pubmed |
| pubmed-article:18295717 | pubmed:abstractText | Polyethylenimine (PEI) cationization is a powerful strategy for protein transduction into cells. In this study, we attempted the artificial regulation of cell proliferation by protein transduction of the N-terminal domain (1-132 amino acids) of the simian virus 40 large T-antigen (SVLT-N), which inactivates retinoblastoma family proteins but not p53. To deliver SVLT-N into cells, we employed an indirect cationization method by forming a complex of biotynylated SVLT-N through disulfide bonds (biotin-SS-SVLT-N) and PEI-cationized avidin (PEI600-avidin). Using this complex, SVLT-N was transduced into the nucleus of confluent and quiescent Balb/c 3T3 cells and was found to be complexed with a cellular target protein, pRb. Furthermore, SVLT-N transduction induced cell proliferation in spite of confluent conditions. Because SVLT-N thus transduced into cells gradually degraded and was not detectable after a 4-d incubation, transiently transformed cells were obtained by this method. These results suggest that oncogene protein transduction technology has great potential for in vitro regulation of cell proliferation. | lld:pubmed |
| pubmed-article:18295717 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18295717 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18295717 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18295717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18295717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18295717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18295717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18295717 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18295717 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:18295717 | pubmed:issn | 1389-1723 | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:KitazoeMidori... | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:TadaHirokoH | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:YamadaHidenor... | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:SenoMasaharuM | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:FutamiJunichi... | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:KosakaMegumiM | lld:pubmed |
| pubmed-article:18295717 | pubmed:author | pubmed-author:MurataHitoshi... | lld:pubmed |
| pubmed-article:18295717 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18295717 | pubmed:volume | 105 | lld:pubmed |
| pubmed-article:18295717 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18295717 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18295717 | pubmed:pagination | 34-8 | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
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| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
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| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:meshHeading | pubmed-meshheading:18295717... | lld:pubmed |
| pubmed-article:18295717 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18295717 | pubmed:articleTitle | Transient cell proliferation with polyethylenimine-cationized N-terminal domain of simian virus 40 large T-antigen. | lld:pubmed |
| pubmed-article:18295717 | pubmed:affiliation | Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan. | lld:pubmed |
| pubmed-article:18295717 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18295717 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
