Linked Life Data

18281344

Source:http://linkedlifedata.com/resource/pubmed/id/18281344

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pubmed-article:18281344pubmed:dateCreated2008-2-18lld:pubmed
pubmed-article:18281344pubmed:abstractTextBlowfly salivary gland cells have a vacuolar-type H(+)-ATPase (V-ATPase) in their apical membrane that energizes secretion of a KCl-rich saliva upon stimulation with serotonin (5-hydroxytryptamine, 5-HT). We have used BCECF to study microfluometrically whether V-ATPase and carbonic anhydrase (CA) are involved in intracellular pH (pH(i)) regulation, and we have localized CA activity by histochemistry. We show: (1) mean pH(i) in salivary gland cells is 7.5+/-0.3 pH units (N=96), higher than that expected from passive H(+) distribution; (2) low 5-HT concentrations (0.3-3 nmol l(-1)) induce a dose-dependent acidification of up to 0.2 pH units, with 5-HT concentrations >10 nmol l(-1), causing monophasic or multiphasic pH changes; (3) the acidifying effect of 5-HT is mimicked by bath application of cAMP, forskolin or IBMX; (4) salivary gland cells exhibit CA activity; (5) CA inhibition with acetazolamide and V-ATPase inhibition with concanamycin A lead to a slow acidification of steady-state pH(i); (6) 5-HT stimuli in the presence of acetazolamide induce an alkalinization that can be decreased by simultaneous application of the V-ATPase inhibitor concanamycin A; (7) concanamycin A removes alkali-going components from multiphasic 5-HT-induced pH changes; (8) NHE activity and a Cl(-)-dependent process are involved in generating 5-HT-induced pH changes; (9) the salivary glands probably contain a Na(+)-driven amino acid transporter. We conclude that V-ATPase and CA contribute to steady-state pH(i) regulation and 5-HT-induced outward H(+) pumping does not cause an alkalinization of pH(i) because of cytosolic H(+) accumulation attributable to stimulated cellular respiration and AE activity, masking the alkalizing effect of V-ATPase-mediated acid extrusion.lld:pubmed
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pubmed-article:18281344pubmed:authorpubmed-author:WalzBerndBlld:pubmed
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pubmed-article:18281344pubmed:volume211lld:pubmed
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pubmed-article:18281344pubmed:year2008lld:pubmed
pubmed-article:18281344pubmed:articleTitleIntracellular pH homeostasis and serotonin-induced pH changes in Calliphora salivary glands: the contribution of V-ATPase and carbonic anhydrase.lld:pubmed
pubmed-article:18281344pubmed:affiliationUniversity of Potsdam, Institute of Biochemistry and Biology, University Campus Golm, Karl-Liebknecht-Strasse 24-25, Potsdam-Golm, Germany.lld:pubmed
pubmed-article:18281344pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:18281344pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:18281344pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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