| pubmed-article:1826917 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1826917 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:1826917 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:1826917 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1826917 | lifeskim:mentions | umls-concept:C0018894 | lld:lifeskim |
| pubmed-article:1826917 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:1826917 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1826917 | pubmed:dateCreated | 1991-5-29 | lld:pubmed |
| pubmed-article:1826917 | pubmed:abstractText | T lymphocytes play an important role in host responses to microbial antigens, and therefore, in order to facilitate studies to determine the mechanisms involved in the regulation of these responses, a method for the generation of T cell clones with specificity to microbial whole cell (WC) antigens has been developed. T cells were purified from the spleen of rats immunized with WC antigen of either the gram-positive bacterium Streptococcus mutans or the gram-negative bacterium Bacteroides gingivalis and cultured with irradiated Sephadex G-10-passed spleen (feeder) cells and the homologous WC antigen for the generation of T cell clones. Clonal growth of the T cells was maintained for up to 6 months by co-culturing similar numbers of T cells, feeder cells and WC antigen; however, the addition of exogenous interleukin-2 (IL-2) was not required. Phenotypic characterization of the cloned T cells showed that they were CD4+ T helper (Th) cells which did not express the leukocyte-common antigen, but did express receptors for 1L-2. These T cells required the presence of homologous WC antigen for growth and their antigen specificity was further confirmed by the ability of the T cells to proliferate in response to the homologous WC, but not to heterologous microbial WC, antigen. These T cells were further characterized for their ability to produce cytokines, specifically 1L-2 and interferon-gamma, and to provide help for B cell responses to microbial WC antigen. This study describes a reproducible method for the generation of rat Th cell clones with specificity to microbial WC antigens which will be valuable for defining the mechanisms involved in T cell regulation of responses to either gram-positive or gram-negative bacteria. | lld:pubmed |
| pubmed-article:1826917 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1826917 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1826917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1826917 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1826917 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1826917 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:1826917 | pubmed:author | pubmed-author:KatoGG | lld:pubmed |
| pubmed-article:1826917 | pubmed:author | pubmed-author:MichalekS MSM | lld:pubmed |
| pubmed-article:1826917 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1826917 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:1826917 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:1826917 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1826917 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1826917 | pubmed:pagination | 77-86 | lld:pubmed |
| pubmed-article:1826917 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:meshHeading | pubmed-meshheading:1826917-... | lld:pubmed |
| pubmed-article:1826917 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1826917 | pubmed:articleTitle | A method for generating antigen-specific rat T helper cell clones. | lld:pubmed |
| pubmed-article:1826917 | pubmed:affiliation | Department of Microbiology, University of Alabama, Birmingham 35294. | lld:pubmed |
| pubmed-article:1826917 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1826917 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1826917 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1826917 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1826917 | lld:pubmed |
