| pubmed-article:18257297 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C0031676 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C0008562 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:18257297 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:18257297 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:18257297 | pubmed:dateCreated | 2008-2-8 | lld:pubmed |
| pubmed-article:18257297 | pubmed:abstractText | Jingzhaotoxin-I (JZTX-I), a 33-residue polypeptide with three disulfide bonds, was a novel spider neurotoxin preferentially inhibiting cardiac sodium channel inactivation. Its activities of phospholipid membrane-binding were studied by a combination of reversed-phase high performance liquid chromatography (HPLC) and fluorescence spectroscopy. Small unilamellar vesicles binding assays showed that the partitioning of JZTX-I into lipid bilayer did not require negatively charged phospholipids. Further, JZTX-I also exhibited a blue shift of 6.4 nm or 4.7 nm as well as red-edge excitation shift of 7.4 nm or 8.0 nm in the presence of 75% 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE )/25% 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG) or 100% 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles respectively, suggesting that some tryptophan residues on the hydrophobic surface of the toxin were located within a motion restricted membrane interfacial region. Fluorescence quenching experiments suggested that some tryptophan residues of JZTX-I were positioned within the membrane and protected from aqueous quenching agents. These findings should provide further insight into the molecular mechanism of the channel gating of JZTX-I. | lld:pubmed |
| pubmed-article:18257297 | pubmed:language | chi | lld:pubmed |
| pubmed-article:18257297 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18257297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18257297 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18257297 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18257297 | pubmed:issn | 1000-8713 | lld:pubmed |
| pubmed-article:18257297 | pubmed:author | pubmed-author:LiangSongping... | lld:pubmed |
| pubmed-article:18257297 | pubmed:author | pubmed-author:ZengXiongzhiX | lld:pubmed |
| pubmed-article:18257297 | pubmed:author | pubmed-author:PiJianhuiJ | lld:pubmed |
| pubmed-article:18257297 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18257297 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:18257297 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18257297 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18257297 | pubmed:pagination | 825-9 | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:meshHeading | pubmed-meshheading:18257297... | lld:pubmed |
| pubmed-article:18257297 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:18257297 | pubmed:articleTitle | [Determination of Jingzhaotoxin-I phospholipid membrane-binding activities by high performance liquid chromatography with fluorescence detection]. | lld:pubmed |
| pubmed-article:18257297 | pubmed:affiliation | The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal University, Changsha 410081, China. xiongzhizeng@yahoo.com.cn | lld:pubmed |
| pubmed-article:18257297 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18257297 | pubmed:publicationType | English Abstract | lld:pubmed |
