Linked Life Data

18255154

Source:http://linkedlifedata.com/resource/pubmed/id/18255154

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SubjectPredicateObjectContext
pubmed-article:18255154rdf:typepubmed:Citationlld:pubmed
pubmed-article:18255154lifeskim:mentionsumls-concept:C0028630lld:lifeskim
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pubmed-article:18255154pubmed:issue4lld:pubmed
pubmed-article:18255154pubmed:dateCreated2008-3-17lld:pubmed
pubmed-article:18255154pubmed:abstractTextCu-induced oxidative damage is associated with cancer, diabetes, neurodegenerative and age related diseases. The quest for Cu-chelators as potential antioxidants spans the past decades. Yet, biocompatible Cu-chelators that do not alter the normal metal-ion homeostasis are still lacking. Here, we explored the potential of natural and synthetic nucleotides and inorganic phosphates as inhibitors of the Cu(I)/(II)-induced ()OH formation via either the Fenton or Haber-Weiss mechanisms. For this purpose, we studied by ESR the modulation of Cu-induced ()OH production, from the decomposition of H(2)O(2), by nucleotides and phosphates. ATP inhibited both Cu(I) and Cu(II) catalyzed reactions (IC(50) 0.11 and 0.04mM, respectively). Likewise, adenosine 5'-beta,gamma-methylene triphosphate (AMP-PCP), adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S), ADP and tripolyphosphate were identified as good inhibitors. However, AMP and adenosine were poor inhibitors in the Cu(I)-H(2)O(2) system, IC(50) ca. 1.2mM, and radical enhancers in the Cu(II)-H(2)O(2) system. The best antioxidant was adenosine 5'-[beta,gamma-imino] triphosphate (AMP-PNP) (IC(50) 0.05mM at Cu(I)-H(2)O(2) system) which was 15 times more active than the known antioxidant Trolox. ATP and analogues inhibit Cu-induced ()OH formation through an ion chelation rather than a scavenging mechanism. Two phosphate groups are required for making active Fenton-reaction inhibitors. Nucleotides and phosphates triggered a biphasic modulation of the Haber-Weiss reaction, but a monophasic inhibition of the Fenton reaction. We conclude that nucleotides at sub mM concentrations can prevent Cu-induced OH radical formation from H(2)O(2), and hence may possibly prevent oxidative damage.lld:pubmed
pubmed-article:18255154pubmed:languageenglld:pubmed
pubmed-article:18255154pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18255154pubmed:citationSubsetIMlld:pubmed
pubmed-article:18255154pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18255154pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18255154pubmed:statusMEDLINElld:pubmed
pubmed-article:18255154pubmed:monthAprlld:pubmed
pubmed-article:18255154pubmed:issn0162-0134lld:pubmed
pubmed-article:18255154pubmed:authorpubmed-author:FischerBilhaBlld:pubmed
pubmed-article:18255154pubmed:authorpubmed-author:Baruch-Suchod...lld:pubmed
pubmed-article:18255154pubmed:issnTypePrintlld:pubmed
pubmed-article:18255154pubmed:volume102lld:pubmed
pubmed-article:18255154pubmed:ownerNLMlld:pubmed
pubmed-article:18255154pubmed:authorsCompleteYlld:pubmed
pubmed-article:18255154pubmed:pagination862-81lld:pubmed
pubmed-article:18255154pubmed:meshHeadingpubmed-meshheading:18255154...lld:pubmed
pubmed-article:18255154pubmed:meshHeadingpubmed-meshheading:18255154...lld:pubmed
pubmed-article:18255154pubmed:meshHeadingpubmed-meshheading:18255154...lld:pubmed
pubmed-article:18255154pubmed:meshHeadingpubmed-meshheading:18255154...lld:pubmed
pubmed-article:18255154pubmed:year2008lld:pubmed
pubmed-article:18255154pubmed:articleTitleCan nucleotides prevent Cu-induced oxidative damage?lld:pubmed
pubmed-article:18255154pubmed:affiliationDepartment of Chemistry, Gonda-Goldschmied Medical Research Center, Bar-Ilan University, Ramat-Gan 52900, Israel.lld:pubmed
pubmed-article:18255154pubmed:publicationTypeJournal Articlelld:pubmed