| pubmed-article:18194271 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18194271 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:18194271 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:18194271 | lifeskim:mentions | umls-concept:C0287638 | lld:lifeskim |
| pubmed-article:18194271 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:18194271 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:18194271 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18194271 | pubmed:dateCreated | 2008-6-19 | lld:pubmed |
| pubmed-article:18194271 | pubmed:abstractText | Toll-like receptors (TLRs) play an important role in innate immunity while, beta(2)-adrenergic receptors (beta(2)AR) provide the key linkages for the sympathetic nervous system to regulate the immune system. However, their role in macrophages remains uncertain. Here, we demonstrate the cross-talk between beta(2)AR and TLR signalling pathways. Expression of beta(2)AR was down-regulated by TLR4 ligand lipopolysaccharide (LPS) stimulation. To investigate the physiological consequence of this down-regulation RAW264 cells, a macrophage cell line, were transfected with a beta(2)AR expression vector (RAWar). Both LPS-stimulated inducible nitric oxide synthase (NOS II) expression and NO production were markedly suppressed in the RAWar cells. The activation of nuclear factor-kappaB (NF-kappaB) and degradation of the inhibitor of NF-kappaB (IkappaBalpha) in response to LPS were markedly decreased in these cells. The level of beta-arrestin 2, which regulates beta(2)AR signalling, was also reduced in RAW264 cells after stimulation with LPS, but not in RAWar cells. Overexpression of beta-arrestin 2 (RAWarr2) also inhibited NO production and NOS II expression. Furthermore, we demonstrated that beta-arrestin 2 interacted with cytosolic IkappaBalpha and that the level of IkappaBalpha coimmunoprecipitated by anti-beta-arrestin 2 antibodies was decreased in the RAW264 cells but not in RAWar or RAWarr2 cells. These findings suggest that LPS-stimulated signals suppress beta(2)AR expression, leading to down-regulation of beta-arrestin 2 expression, which stabilizes cytosolic IkappaBalpha and inhibits the NF-kappaB activation essential for NOS II expression, probably to ensure rapid and sufficient production of NO in response to microbial attack. | lld:pubmed |
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| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18194271 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18194271 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18194271 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:18194271 | pubmed:issn | 1365-2567 | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:TaniguchiNaoy... | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:TobaKenjiK | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:WatanabeKenji... | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:TajiriHisaoH | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:DayNoorbibi... | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:SakuraiTakuya... | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:KizakiTakakoT | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:OhnoHidekiH | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:IzawaTetsuyaT | lld:pubmed |
| pubmed-article:18194271 | pubmed:author | pubmed-author:HagaShukohS | lld:pubmed |
| pubmed-article:18194271 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18194271 | pubmed:volume | 124 | lld:pubmed |
| pubmed-article:18194271 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18194271 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18194271 | pubmed:pagination | 348-56 | lld:pubmed |
| pubmed-article:18194271 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
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| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
| pubmed-article:18194271 | pubmed:meshHeading | pubmed-meshheading:18194271... | lld:pubmed |
| pubmed-article:18194271 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18194271 | pubmed:articleTitle | Beta2-adrenergic receptor regulates Toll-like receptor-4-induced nuclear factor-kappaB activation through beta-arrestin 2. | lld:pubmed |
| pubmed-article:18194271 | pubmed:affiliation | Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Japan. kizaki@kyorin-u.ac.jp | lld:pubmed |
| pubmed-article:18194271 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18194271 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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