| pubmed-article:18160177 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18160177 | lifeskim:mentions | umls-concept:C1705994 | lld:lifeskim |
| pubmed-article:18160177 | lifeskim:mentions | umls-concept:C1513492 | lld:lifeskim |
| pubmed-article:18160177 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:18160177 | lifeskim:mentions | umls-concept:C0086149 | lld:lifeskim |
| pubmed-article:18160177 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18160177 | pubmed:dateCreated | 2008-3-24 | lld:pubmed |
| pubmed-article:18160177 | pubmed:abstractText | The amoeba Dictyostelium discoideum possesses genes for 13 different kinesins. Here we characterize DdKif3, a member of the Kinesin-1 family. Kinesin-1 motors form homodimers that can move micrometer-long distances on microtubules using the energy derived from ATP hydrolysis. We expressed recombinant motors in Escherichia coli and tested them in different in vitro assays. Full-length and truncated Kif3 motors were active in gliding and ATPase assays. They showed a strong dependence on ionic strength. Like the full-length motor, the truncated DdKif3-592 motor (aa 1-592; comprising motor domain, neck, and partial stalk) reached its maximum speed of around 2.0micrcom s(-1) at a potassium acetate concentration of 200mM. The shortened DdKif3-342 motor (aa 1-342; comprising motor domain, partial neck) showed a high ATP turnover, comparable to that of the fungal Kinesin-1, Nkin. Results from the duty cycle calculations and gliding assays indicate that DdKif3 is a processive motor. A GFP-fusion protein revealed a mainly cytoplasmic localization of DdKif3. Immunofluorescence staining makes an association with the endoplasmic reticulum or mitochondria unlikely. Despite a similar phylogenetic distance to both metazoa and fungi, in terms of its biochemical properties DdKif3 revealed a closer similarity to fungal than animal kinesins. | lld:pubmed |
| pubmed-article:18160177 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18160177 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18160177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18160177 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18160177 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18160177 | pubmed:issn | 0171-9335 | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:SchliwaManfre... | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:LiuXiaoX | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:WoehlkeGünthe... | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:ParschJohnJ | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:RohlfsMeinoM | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:LeierSvenS | lld:pubmed |
| pubmed-article:18160177 | pubmed:author | pubmed-author:RöhlkChristia... | lld:pubmed |
| pubmed-article:18160177 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18160177 | pubmed:volume | 87 | lld:pubmed |
| pubmed-article:18160177 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18160177 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18160177 | pubmed:pagination | 237-49 | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
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| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:meshHeading | pubmed-meshheading:18160177... | lld:pubmed |
| pubmed-article:18160177 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18160177 | pubmed:articleTitle | Properties of the Kinesin-1 motor DdKif3 from Dictyostelium discoideum. | lld:pubmed |
| pubmed-article:18160177 | pubmed:affiliation | Department of Physics, Technical University Munich, James-Franck-Strasse, D-85748 Garching, Germany. | lld:pubmed |
| pubmed-article:18160177 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18160177 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:8622903 | entrezgene:pubmed | pubmed-article:18160177 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18160177 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18160177 | lld:pubmed |
