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pubmed-article:1815956pubmed:abstractTextAn electrophoretic method (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is described which permits the identification of human leukocyte antigen HLA-DQA1 types and subtypes without using allele-specific oligonucleotide probes and dot-blot methodology. The procedure can be used in miniaturized gels in combination with automated electrophoretic systems. The PhastSystem is particularly recommended since temperature control is essential. HLA-DQA1*01 and DQA1*0301 can be distinguished in homoduplexes and DQA1*01 subtypes, DQA1*0201 and DQA1*0401 in heteroduplexes (in only 5 h including DNA extraction and PCR amplification). Additional variations to those recognized using commercially available dot-blot methods can be provided since this procedure permits the identification of single base-pair substitutions. In addition, this method is faster and less expensive than commercial methods.lld:pubmed
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pubmed-article:1815956pubmed:authorpubmed-author:CarracedoAAlld:pubmed
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pubmed-article:1815956pubmed:pagination1041-5lld:pubmed
pubmed-article:1815956pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:1815956pubmed:articleTitleElectrophoretic human leukocyte antigen HLA-DQA1 DNA typing after polymerase chain reaction amplification.lld:pubmed
pubmed-article:1815956pubmed:affiliationDepartment of Legal Medicine, Faculty of Medicine, Santiago de Compostela, Spain.lld:pubmed
pubmed-article:1815956pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1815956pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed