pubmed-article:1815956 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1815956 | lifeskim:mentions | umls-concept:C0019721 | lld:lifeskim |
pubmed-article:1815956 | lifeskim:mentions | umls-concept:C0079247 | lld:lifeskim |
pubmed-article:1815956 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
pubmed-article:1815956 | lifeskim:mentions | umls-concept:C0122013 | lld:lifeskim |
pubmed-article:1815956 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
pubmed-article:1815956 | pubmed:issue | 12 | lld:pubmed |
pubmed-article:1815956 | pubmed:dateCreated | 1992-6-26 | lld:pubmed |
pubmed-article:1815956 | pubmed:abstractText | An electrophoretic method (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is described which permits the identification of human leukocyte antigen HLA-DQA1 types and subtypes without using allele-specific oligonucleotide probes and dot-blot methodology. The procedure can be used in miniaturized gels in combination with automated electrophoretic systems. The PhastSystem is particularly recommended since temperature control is essential. HLA-DQA1*01 and DQA1*0301 can be distinguished in homoduplexes and DQA1*01 subtypes, DQA1*0201 and DQA1*0401 in heteroduplexes (in only 5 h including DNA extraction and PCR amplification). Additional variations to those recognized using commercially available dot-blot methods can be provided since this procedure permits the identification of single base-pair substitutions. In addition, this method is faster and less expensive than commercial methods. | lld:pubmed |
pubmed-article:1815956 | pubmed:language | eng | lld:pubmed |
pubmed-article:1815956 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1815956 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1815956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1815956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1815956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1815956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1815956 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1815956 | pubmed:month | Dec | lld:pubmed |
pubmed-article:1815956 | pubmed:issn | 0173-0835 | lld:pubmed |
pubmed-article:1815956 | pubmed:author | pubmed-author:BarrowBB | lld:pubmed |
pubmed-article:1815956 | pubmed:author | pubmed-author:CarracedoAA | lld:pubmed |
pubmed-article:1815956 | pubmed:author | pubmed-author:Rodriguez-Cal... | lld:pubmed |
pubmed-article:1815956 | pubmed:author | pubmed-author:LareuM VMV | lld:pubmed |
pubmed-article:1815956 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1815956 | pubmed:volume | 12 | lld:pubmed |
pubmed-article:1815956 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1815956 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1815956 | pubmed:pagination | 1041-5 | lld:pubmed |
pubmed-article:1815956 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
pubmed-article:1815956 | pubmed:meshHeading | pubmed-meshheading:1815956-... | lld:pubmed |
pubmed-article:1815956 | pubmed:meshHeading | pubmed-meshheading:1815956-... | lld:pubmed |
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pubmed-article:1815956 | pubmed:meshHeading | pubmed-meshheading:1815956-... | lld:pubmed |
pubmed-article:1815956 | pubmed:meshHeading | pubmed-meshheading:1815956-... | lld:pubmed |
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pubmed-article:1815956 | pubmed:meshHeading | pubmed-meshheading:1815956-... | lld:pubmed |
pubmed-article:1815956 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:1815956 | pubmed:articleTitle | Electrophoretic human leukocyte antigen HLA-DQA1 DNA typing after polymerase chain reaction amplification. | lld:pubmed |
pubmed-article:1815956 | pubmed:affiliation | Department of Legal Medicine, Faculty of Medicine, Santiago de Compostela, Spain. | lld:pubmed |
pubmed-article:1815956 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1815956 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |