| pubmed-article:18084979 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C1419778 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C1742737 | lld:lifeskim |
| pubmed-article:18084979 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:18084979 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:18084979 | pubmed:dateCreated | 2007-12-18 | lld:pubmed |
| pubmed-article:18084979 | pubmed:abstractText | Malignant hyperthermia is a pharmacogenetic disorder caused by autosomal dominant mutations in the ryanodine receptor type 1 gene. Propofol has been reported as a safe anaesthetic for malignant hyperthermia susceptible patients but has not been tested on cultured cells from patients with the ryanodine receptor type 1 mutation. The aim of this study was to determine whether propofol could trigger abnormal calcium fluxes in human myotubes isolated from malignant hyperthermia susceptible patients harbouring the native ryanodine receptor type 1 mutation. Muscle specimens were obtained from the patients to diagnose malignant hyperthermia disposition and the calcium-induced calcium release test and molecular genetic analyses were performed. Using the calcium sensitive probe Fura 2, we determined the 340/380 nm wave-length ratios by measuring alterations in calcium homeostasis in isolated myotubes from cultured skeletal muscle specimens. Two patients, one with ryanodine receptor type 1 R2508C and one with the L4838V mutation had accelerated calcium-induced calcium release rates. The 340/380 nm ratios increased when the propofol concentration exceeded 100 microM. The half-maximal activation concentrations (EC50) for propofol from patients 1 and 2 were 181.1 and 420.5 microM, respectively. Increases in calcium concentrations in response to propofol dosage were limited to doses at least 100-fold greater than those used in clinical settings. These observations correlate well with clinical observations that propofol does not trigger malignant hyperthermia in susceptible humans. | lld:pubmed |
| pubmed-article:18084979 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18084979 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18084979 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18084979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18084979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18084979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18084979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18084979 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18084979 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:18084979 | pubmed:issn | 0310-057X | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:KobayashiMM | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:KawamotoMM | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:MigitaTT | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:NishinoII | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:MukaidaKK | lld:pubmed |
| pubmed-article:18084979 | pubmed:author | pubmed-author:YugetOO | lld:pubmed |
| pubmed-article:18084979 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18084979 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:18084979 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18084979 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18084979 | pubmed:pagination | 894-8 | lld:pubmed |
| pubmed-article:18084979 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
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| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
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| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:meshHeading | pubmed-meshheading:18084979... | lld:pubmed |
| pubmed-article:18084979 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:18084979 | pubmed:articleTitle | Propofol-induced changes in myoplasmic calcium concentrations in cultured human skeletal muscles from RYR1 mutation carriers. | lld:pubmed |
| pubmed-article:18084979 | pubmed:affiliation | Department of Anesthesiology and Critical Care, Hiroshima University, Hiroshima, Japan. | lld:pubmed |
| pubmed-article:18084979 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18084979 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
