| pubmed-article:18065398 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0009325 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0014144 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0596508 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0007585 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0050668 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0017243 | lld:lifeskim |
| pubmed-article:18065398 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:18065398 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18065398 | pubmed:dateCreated | 2008-2-18 | lld:pubmed |
| pubmed-article:18065398 | pubmed:abstractText | Embryonic stem cell-derived endoderm is critical for the development of cellular therapies for the treatment of disease such as diabetes, liver cirrhosis, or pulmonary emphysema. Here, we describe a novel approach to induce endoderm from mouse embryonic stem (mES) cells using fibronectin-coated collagen gels. This technique results in a homogeneous endoderm-like cell population, demonstrating endoderm-specific gene and protein expression, which remains committed following in vivo transplantation. In this system, activin, normally an endoderm inducer, caused an 80% decrease in the Foxa2-positive endoderm fraction, whereas follistatin increased the Foxa2-positive endoderm fraction to 78%. Our work suggests that activin delays the induction of endoderm through its transient precursors, the epiblast and mesendoderm. Long-term differentiation displays a twofold reduction in hepatic gene expression and threefold reduction in hepatic protein expression of activin-treated cells compared with follistatin-treated cells. Moreover, subcutaneous transplantation of activin-treated cells in a syngeneic mouse generated a heterogeneous teratoma-like mass, suggesting that these were a more primitive population. In contrast, follistatin-treated cells resulted in an encapsulated epithelial-like mass, suggesting that these cells remained committed to the endoderm lineage. In conclusion, we demonstrate a novel technique to induce the direct differentiation of endoderm from mES cells without cell sorting. In addition, our work suggests a new role for activin in induction of the precursors to endoderm and a new endoderm-enrichment technique using follistatin. | lld:pubmed |
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| pubmed-article:18065398 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18065398 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18065398 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:18065398 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18065398 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:18065398 | pubmed:issn | 1549-4918 | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:YarmushMartin... | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:BerthiaumeFra... | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:TillesArno... | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:ParashuramaNa... | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:NahmiasYaakov... | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:van PollDaanD | lld:pubmed |
| pubmed-article:18065398 | pubmed:author | pubmed-author:ChoCheul HCH | lld:pubmed |
| pubmed-article:18065398 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18065398 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:18065398 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18065398 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18065398 | pubmed:pagination | 474-84 | lld:pubmed |
| pubmed-article:18065398 | pubmed:dateRevised | 2010-12-3 | lld:pubmed |
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