| pubmed-article:18060735 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C0009063 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C0596402 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:18060735 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:18060735 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18060735 | pubmed:dateCreated | 2008-3-24 | lld:pubmed |
| pubmed-article:18060735 | pubmed:abstractText | Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). We investigated the role of the conserved Ca(2+)-binding motif of Ib in the cytotoxicity of iota-toxin. The cytotoxicity of iota-toxin increased with an increase in the concentration of extracellular Ca(2+). A surface plasmon resonance analysis showed that the binding of Ia to the oligomer of Ib is dependent on the concentration of Ca(2+). However, the addition of Ca(2+) had no effect on the binding of (125)I-labeled Ib to the cells. We replaced Asp-8, -10, and -12 in the Ca(2+)-binding motif of Ib with alanine. D8A, D10A, and D12A bound to the cell and formed an oligomer at about half of the wild-type Ib. The cytotoxicity of Ib variants in the presence of Ia was about 500-fold less than that of wild-type Ib. Immunofluorescence study showed that these variants were internalized in the early endosomes like wild-type Ib. However, wild-type Ib-induced internalization of Ia in the cells, but these variants did not. The result indicates that the conserved Ca(2+)-binding motif in the N-terminal region of Ib plays a role in the interaction of Ib with Ia in the presence of Ca(2+). | lld:pubmed |
| pubmed-article:18060735 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18060735 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18060735 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18060735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18060735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18060735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18060735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18060735 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18060735 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18060735 | pubmed:issn | 0882-4010 | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:KojimaTakashi... | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:KobayashiKeik... | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:OdaMasatakaM | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:NagahamaMasah... | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:SakuraiJunJ | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:OhkuboNorikoN | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:ShiraiHidenor... | lld:pubmed |
| pubmed-article:18060735 | pubmed:author | pubmed-author:IwamotoShinob... | lld:pubmed |
| pubmed-article:18060735 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18060735 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:18060735 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18060735 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18060735 | pubmed:pagination | 265-70 | lld:pubmed |
| pubmed-article:18060735 | pubmed:meshHeading | pubmed-meshheading:18060735... | lld:pubmed |
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| pubmed-article:18060735 | pubmed:meshHeading | pubmed-meshheading:18060735... | lld:pubmed |
| pubmed-article:18060735 | pubmed:meshHeading | pubmed-meshheading:18060735... | lld:pubmed |
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| pubmed-article:18060735 | pubmed:meshHeading | pubmed-meshheading:18060735... | lld:pubmed |
| pubmed-article:18060735 | pubmed:meshHeading | pubmed-meshheading:18060735... | lld:pubmed |
| pubmed-article:18060735 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18060735 | pubmed:articleTitle | Role of Ca2+-binding motif in cytotoxicity induced by Clostridium perfringens iota-toxin. | lld:pubmed |
| pubmed-article:18060735 | pubmed:affiliation | Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima 7708514, Japan. | lld:pubmed |
| pubmed-article:18060735 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18060735 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
