| pubmed-article:18025283 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0025202 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0242640 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C1527249 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C0385569 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C1555029 | lld:lifeskim |
| pubmed-article:18025283 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:18025283 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:18025283 | pubmed:dateCreated | 2007-11-20 | lld:pubmed |
| pubmed-article:18025283 | pubmed:abstractText | Overexpression of the multidrug resistance 1 (MDR1) gene, encoding P-glycoprotein (P-gp), facilitates resistance to diverse chemotherapeutic drugs and current P-gp inhibitors display high toxicity. We studied the effects of melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24), which exhibits cancer-specific apoptosis-inducing properties, in drug-sensitive (SW620) and drug-resistant (SW620/Dox) colorectal carcinoma cells. Adenovirus administered mda-7/IL-24, Ad.mda-7, effectively reversed resistance to doxorubicin-induced apoptosis in SW620/Dox cells by increased intracellular accumulation and decreased efflux of doxorubicin. Unexpectedly, P-gp-overexpressing cells (SW620/Dox) displayed increased apoptosis following Ad.mda-7 infection compared with parental SW620 cells, which correlated with more MDA-7/IL-24 protein in SW620/Dox than SW620 cell and potentially explains the increased sensitivity of P-gp-overexpressing cells to mda-7/IL-24. Transient overexpression of MDR1 in SW620 cells significantly increased apoptosis, decreased anchorage-independent growth, and increased MDA-7/IL-24 protein following Ad.mda-7 infection, whereas down-modulation of MDR1 in SW620/Dox cells by small interfering RNA decreased apoptosis following Ad.mda-7 infection. The increased mda-7/IL-24 sensitivity observed in SW620/Dox cells was partly due to increased reactive oxygen species generation and lower mitochondrial membrane potential. These findings confirm that mda-7/IL-24 is a potent MDR reversal agent, preferentially causing apoptosis in P-gp-overexpressing MDR cells, suggesting significant expanded clinical implications for the use of mda-7/IL-24 in treating neoplasms that have failed chemotherapy mediated by the P-gp MDR mechanism. | lld:pubmed |
| pubmed-article:18025283 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18025283 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18025283 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18025283 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18025283 | pubmed:issn | 1535-7163 | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:DentPaulP | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:CurielDavid... | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:FisherPaul... | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:LebedevaIrina... | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:SuZao-ZhongZZ | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:SarkarDevanan... | lld:pubmed |
| pubmed-article:18025283 | pubmed:author | pubmed-author:EmdadLuniL | lld:pubmed |
| pubmed-article:18025283 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18025283 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:18025283 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18025283 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18025283 | pubmed:pagination | 2985-94 | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:meshHeading | pubmed-meshheading:18025283... | lld:pubmed |
| pubmed-article:18025283 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:18025283 | pubmed:articleTitle | Melanoma differentiation associated gene-7/interleukin-24 reverses multidrug resistance in human colorectal cancer cells. | lld:pubmed |
| pubmed-article:18025283 | pubmed:affiliation | Department of Urology, Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY 10032, USA. | lld:pubmed |
| pubmed-article:18025283 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18025283 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18025283 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:11009 | entrezgene:pubmed | pubmed-article:18025283 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:18025283 | lld:entrezgene |
